| Autor: |
Izdebski, R. Fiett, J. Urbanowicz, P. Baraniak, A. Derde, L.P.G. Bonten, M.J.M. Carmeli, Y. Goossens, H. Hryniewicz, W. Brun-Buisson, C. Brisse, S. Gniadkowski, M. Herda, M. Dautzenberg, M.J. Adler, A. Kazma, M. Navon-Venezia, S. Malhotra-Kumar, S. Lammens, C. Legrand, P. Chalfine, A. Giamarellou, H. Petrikkos, G.L. Balode, A. Dumpis, U. Stammet, P. Aragăo, I. Esteves, F. Torres Martí, A. Lawrence, C. Salomon, J. Paul, M. Lerman, Y. Rossini, A. Salvia, A. Vidal Samso, J. Fierro, J. MOSAR WP2, WP3 WP5 Study Groups |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Popis: |
Objectives: The objective of this study was to examine Klebsiella oxytoca clonal and phylogenetic diversity, based on an international collection of carriage isolates non-susceptible to expanded-spectrum cephalosporins (ESCs). Methods: The study material comprised 68 rectal carriage K. oxytoca isolates non-susceptible to ESCs recovered in 2008-11 from patients in 14 hospitals across Europe and Israel. ESC resistance was tested phenotypically; genes encoding ESBLs, AmpC cephalosporinases and carbapenemases were amplified and sequenced. The isolates were typed by PFGE and MLST, followed by sequencing of blaOXY genes. Results: MLSTand PFGE distinguished 34 STs and 47 pulsotypes among the isolates, respectively. Six STswere split into several pulsotypes each. Five STs were more prevalent (n=2-9) and occurred in several countries each, including ST2, ST9 and ST141, which belong to a growing international clonal complex (CC), CC2. Four phylogenetic lineages were distinguished, each with another type of chromosomal OXY-type β-lactamase. Three of these, with OXY-1/-5, OXY-2 types and OXY-4, corresponded to previously described phylogroups KoI, KoII and KoIV, respectively. A single isolate from Israel represented a distinct lineage with a newly defined OXY-7 type. The phylogroups showed interesting differences in mechanisms of ESC resistance; KoI strains rarely overexpressed the OXY enzymes but commonly produced ESBLs, whereas KoII strains often were OXY hyperproducers and carried ESBLs much less frequently. AmpCs (DHA-1) and carbapenemases (VIM-1) occurred sporadically. Conclusions: The study confirmed the high genetic diversity of the collection of K. oxytoca ESC-non-susceptible isolates, composed of phylogroups with distinct types of OXY-type β-lactamases, and revealed some STs of broad geographical distribution. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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