Evaluation of the clinical sensitivity for the quantification of human immunodeficiency virus type 1 RNA in plasma: Comparison of the new COBAS TaqMan HIV-1 with three current HIV-RNA assays-LCx HIV RNA quantitative, VERSANT HIV-1 RNA 3.0 (bDNA) and COBAS AMPLICOR HIV-1 Monitor v1.5
Autor: | Katsoulidou, A Petrodaskalaki, M Sypsa, V Papachristou, E and Anastassopoulou, CG Gargalianos, P Karafoulidou, A and Lazanas, M Kordossis, T Andoniadou, A Hatzakis, A |
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Jazyk: | angličtina |
Rok vydání: | 2006 |
Předmět: | |
Popis: | The COBAS TaqMan HIV-1 test (Roche Diagnostics) was compared with the LCx HIV RNA quantitative assay (Abbott Laboratories), the Versant HIV-1 RNA 3.0 (bDNA) assay (Bayer) and the COBAS Amplicor HIV-1 Monitor v1.5 test (Roche Diagnostics), using plasma samples of various viral load levels from HIV-1-infected individuals. In the comparison of TaqMan with LCx, TaqMan identified as positive 77.5% of the 240 samples versus 72.1% identified by LCx assay, while their overall agreement was 94.6% and the quantitative results of samples that were positive by both methods were strongly correlated (r = 0.91). Similarly, in the comparison of TaqMan with bDNA 3.0, both methods identified 76.3% of the 177 samples as positive, while their overall agreement was 95.5% and the quantitative results of samples that were positive by both methods were strongly correlated (r = 0.95). Finally, in the comparison of TaqMan with Monitor v 1.5, TaqMan identified 79.5% of the 156 samples as positive versus 80.1% identified by Monitor v 1.5, while their overall agreement was 95.5% and the quantitative results of samples that were positive by both methods were strongly correlated (r = 0.96). In conclusion, the new COBAS TaqMan HIV-1 test showed excellent agreement with other widely used commercially available tests for the quantitation of HIV-1 viral load. (c) 2005 Elsevier B.V. All rights reserved. |
Databáze: | OpenAIRE |
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