| Autor: |
Terpos, E. Politou, M. Sergentanis, T.N. Mentis, A. Rosati, M. Stellas, D. Bear, J. Hu, X. Felber, B.K. Pappa, V. Pagoni, M. Grouzi, E. Labropoulou, S. Charitaki, I. Ntanasis-Stathopoulos, I. Moschandreou, D. Bouhla, A. Saridakis, S. Korompoki, E. Giatra, C. Bagratuni, T. Pefanis, A. Papageorgiou, S. Spyridonidis, A. Antoniadou, A. Kotanidou, A. Syrigos, K. Stamoulis, K. Panayiotakopoulos, G. Tsiodras, S. Alexopoulos, L. Dimopoulos, M.A. Pavlakis, G.N. |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Popis: |
We evaluated the antibody responses in 259 potential convalescent plasma donors for Covid-19 patients. Different assays were used: a commercial ELISA detecting antibodies against the recombinant spike protein (S1); a multiplex assay detecting total and specific antibody isotypes against three SARS-CoV-2 antigens (S1, basic nucleocapsid (N) protein and receptor-binding domain (RBD)); and an in-house ELISA detecting antibodies to complete spike, RBD and N in 60 of these donors. Neutralizing antibodies (NAb) were also evaluated in these 60 donors. Analyzed samples were collected at a median time of 62 (14–104) days from the day of first symptoms or positive PCR (for asymptomatic patients). Anti-SARS-CoV-2 antibodies were detected in 88% and 87.8% of donors using the ELISA and the multiplex assay, respectively. The multivariate analysis showed that age ≥50 years (p < 0.001) and need for hospitalization (p < 0.001) correlated with higher antibody titers, while asymptomatic status (p < 0.001) and testing >60 days after symptom onset (p = 0.001) correlated with lower titers. Interestingly, pseudotype virus-neutralizing antibodies (PsNAbs) significantly correlated with spike and with RBD antibodies by ELISA. Sera with high PsNAb also showed a strong ability to neutralize active SARS-CoV-2 virus, with hospitalized patients showing higher titers. Therefore, convalescent plasma donors can be selected based on the presence of high RBD antibody titers. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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