| Autor: |
Khojasteh, Arash, Fahimipour, Farahnaz, Jafarian, Mohammad, Sharifi, Davoud, Jahangir, Shahrbanoo, Khayyatan, Fahimeh, Eslaminejad, Mohamadreza Baghaban |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Journal of biomedical materials research: part B : applied biomaterials |
| ISSN: |
1552-4973 |
| Popis: |
We sought to assess the effects of coculturing mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) in the repair of dog mandible bone defects. The cells were delivered in -tricalcium phosphate scaffolds coated with poly lactic co-glycolic acid microspheres that gradually release vascular endothelial growth factor (VEGF). The complete scaffold and five partial scaffolds were implanted in bilateral mandibular body defects in eight beagles. The scaffolds were examined histologically and morphometrically 8weeks after implantation. Histologic staining of the decalcified scaffolds demonstrated that bone formation was greatest in the VEGF/MSC scaffold (63.42 +/- 1.67), followed by the VEGF/MSC/EPC (47.8 +/- 1.87) and MSC/EPC (45.21 +/- 1.6) scaffolds, the MSC scaffold (34.59 +/- 1.49), the VEGF scaffold (20.03 +/- 1.29), and the untreated scaffold (7.24 +/- 0.08). Hence, the rate of new bone regeneration was highest in scaffolds containing MSC, either mixed with EPC or incorporating VEGF. Adding both EPC and VEGF with the MSC was not necessary. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1767-1777, 2017. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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