| Autor: |
Manageiro, Vera, Cano, Manuela, Dias, Elsa, Rosado, Tânia, Vieira, Patrícia, Reis, Lígia, Matias, Rui, Rodrigues, João, Coelho, Rodrigo, Menezes, Carina, Ferreira, Eugénia, Sequeira, António, Moreira, Olga, Caniça, Manuela |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Popis: |
Aim: The aim of this study was to determine the microbial biodiversity and naturally occurring antibiotic resistance (AR)-encoding genes background load along the food/feed chain in an open-air agricultural testing catchment (HOAL). Methods: Samples were collected in two time points during the year 2020 from various – but interconnected – environmental compartments of the food/feed chain within an HOAL catchment, such as air, pig feces, manure, soil, water, crops, and feed. All samples were homogenized, diluted and plated in selective media. Colonies were selected andidentification made by MALDI-TOF. Antibiotic susceptibility (AST) was assessed by disc diffusion and/or MIC methods. DNA was extracted for further molecular and genomic characterization. Results: A total of 753 Gram-negative strains were isolated. The highest number of bacteria were from families of Enterobacteriaceae(44.1%), Morganellaceae(15.5%), Moraxellaceae(15.5%), and Pseudomonadaceae(9.3%). Enterobacteriaceae and Pseudomonadaceae were present in all but one of the tested compartments (ground water and feed, respectively). Of notice, 43.1% of Enterobacteriaceae identified were isolated from a selective medium supplemented with colistin 0.5mg/L and 8.7% were selected with cefotaxime 2mg/L. Extended-spectrum -lactamase (ESBL)-producing strains were identified. Regarding Gram-positive bacteria, we identified mainly Enterococcaceae (46.7%), Bacillaceae (30.6%), and Streptococcaceae (13.9%). Pig faces and manure were the compartments with higher bacterial biodiversity. Phenotypic AST revealed the presence of resistances against several AR classes. Based on this data, strains were classified as multidrug-resistant. Conclusions: The investigation of clinically important AR-encoding genes by PCR-amplification and whole-genome-sequencing of selected strains will contribute to clarify the resistome and microbial biodiversity in the tested environmental compartments. This project is funded by One Health EJP info:eu-repo/semantics/publishedVersion |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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