Review of the impact of MALDI-TOF MS in public health and hospital hygiene, 2018
| Autor: | Rodríguez-Sánchez, B., Cercenado, E., Coste, A.T., Greub, G. |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Anti-Bacterial Agents/therapeutic use
Bacteremia/diagnosis Bacteremia/drug therapy Bacteremia/microbiology Bacteria/classification Bacteria/drug effects Bacteria/isolation & purification Drug Resistance Microbial Humans Hygiene Microbial Sensitivity Tests/methods Microbiological Techniques/methods Mycological Typing Techniques Public Health Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization/methods MALDI-TOF MS Mass Spectrometry antibiotic resistance mechanisms antibiotic use hygiene microorganism identification mycobacteria typing |
| Zdroj: | Euro surveillance, vol. 24, no. 4 |
| Popis: | IntroductionMALDI-TOF MS represents a new technological era for microbiology laboratories. Improved sample processing and expanded databases have facilitated rapid and direct identification of microorganisms from some clinical samples. Automated analysis of protein spectra from different microbial populations is emerging as a potential tool for epidemiological studies and is expected to impact public health.AimTo demonstrate how implementation of MALDI-TOF MS has changed the way microorganisms are identified, how its applications keep increasing and its impact on public health and hospital hygiene.MethodsA review of the available literature in PubMED, published between 2009 and 2018, was carried out.ResultsOf 9,709 articles retrieved, 108 were included in the review. They show that rapid identification of a growing number of microorganisms using MALDI-TOF MS has allowed for optimisation of patient management through prompt initiation of directed antimicrobial treatment. The diagnosis of Gram-negative bacteraemia directly from blood culture pellets has positively impacted antibiotic streamlining, length of hospital stay and costs per patient. The flexibility of MALDI-TOF MS has encouraged new forms of use, such as detecting antibiotic resistance mechanisms (e.g. carbapenemases), which provides valuable information in a reduced turnaround time. MALDI-TOF MS has also been successfully applied to bacterial typing.ConclusionsMALDI-TOF MS is a powerful method for protein analysis. The increase in speed of pathogen detection enables improvement of antimicrobial therapy, infection prevention and control measures leading to positive impact on public health. For antibiotic susceptibility testing and bacterial typing, it represents a rapid alternative to time-consuming conventional techniques. |
| Databáze: | OpenAIRE |
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