| Autor: |
Mariani, Paolo, Spinozzi, Francesco, Federiconi, Francesco, Ortore, M. G., Amenitsch, Heinz, Spindler, Lea, Drevenšek Olenik, Irena |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
G-Quadruplex Nucleic Acids, Journal of Nucleic Acids, vol. 2010, pp. 1-10, 2010. |
| ISSN: |
2090-021X |
| Popis: |
We investigated quadruplex formation in aqueous solutions of -deoxyriboguanosine -monophosphate, d(pG), which takes place in the absence of the covalent axial backbone. A series of in-solution small angle X-ray scattering experiments on d(pG) have been performed as a function of temperature in the absence of excess salt, at a concentration just above the critical one at which self-assembling occurs. A global fit approach has been used to derive composition and size distribution of the scattering particles as a function of temperature. The obtained results give thermodynamical justification for the observed phase-behavior, indicating that octamer formation is essential for quadruplex elongation. Our investigation shows that d(pG) quadruplexes are very suitable to assess the potential of G-quadruplex formation and to study the self-assembling thermodynamics. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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