Event-specific Method for the Quantification of Maize Event Bt11 Using Real-time PCR
| Autor: | CHARLES DELOBEL Chrystele, LARCHER Sara, MAZZARA Marco, VAN DEN EEDE Guy |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Popis: | The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the Bt11 transformation event in maize DNA (unique identifier SYN-BTØ11-1). The collaborative trial was conducted according to internationally accepted guidelines (1, 2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Syngenta Seeds S.A.S. provided the detection method and the samples (genomic DNA of conventional maize and of maize event Bt11). The JRC prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from nine European countries. The results of the international collaborative trial met the ENGL performance requirements. The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004. JRC.I.6-Biotechnology and GMOs |
| Databáze: | OpenAIRE |
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