Detection of classical and atypical/Nor98 scrapie by the paraffin-embedded tissue blot method
| Autor: | Wemheuer, W. M., Benestad, S. L., Wrede, A., Wemheuer, W. E., Brenig, B., Bratberg, B., Schulz-Schaeffer, W. J. |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Central Nervous System
Paraffin Embedding Sheep Lymphoid Tissue Prions Blotting Western Palatine Tonsil Brain Immunohistochemistry Sensitivity and Specificity Animals Blotting Western/methods Blotting Western/veterinary Brain/pathology Case-Control Studies Central Nervous System/pathology Immunohistochemistry/veterinary Lymphoid Tissue/pathology Palatine Tonsil/pathology Paraffin Embedding/methods Prions/genetics Prions/isolation & purification Scrapie/genetics Scrapie/pathology Scrapie |
| Popis: | The paraffin-embedded tissue (PET) blot method was used to investigate sections of the central nervous system and lymphatic tissues from 24 cases of classical scrapie and 25 cases of atypical/Nor98 scrapie in sheep and four healthy control sheep. The PET blot detected deposits of PrP(Sc) in the brain tissue of all 49 sheep with scrapie but no PrP(Sc) labelling could be detected in the control sheep. By contrast, not all the atypical/Nor98 scrapie cases were detectable by immunohistochemistry. The high sensitivity of the PET blot method made it possible to observe that in some atypical/Nor98 cases, deposits of PrP(Sc) may be restricted to supratentorial brain structures and that the diagnosis may be missed when only testing the obex area, where deposits are common in classical scrapie, and the cerebellar structures, where deposits are considered to be common in atypical/Nor98 cases. peerReviewed |
| Databáze: | OpenAIRE |
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