The structure of the exocellular polysaccharide produced by Rhodococcus sp. RHA1
| Autor: | Perry, Malcolm, MacLean, Leann, Patrauchan, M., Vinogradov, Evguenii |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Canada
spectroscopy models chemical spectra galactose magnetic resonance spectroscopy NMR NMR spectroscopy solvents fucose hydrolysis oligosaccharides carbohydrate conformation polysaccharide rhodococcus chromatography acid methylation structure glucose metabolism 2D D-glucose polymers glucuronic acid |
| Popis: | Rhodococcus sp. RHA1 is a Gram-positive actinomycete capable of metabolizing a wide spectrum of organic compounds whose survival in chemically hostile environments is believed to be in part due to the production of an exocellular polysaccharide (EPS). In order to investigate the functional nature of the EPS, its structure was determined using a combinatory approach including hydrolysis, composition, and methylation, analysis methods, as well as 2D (1)H and (13)C NMR spectroscopy. The EPS was found to be a high-molecular-mass polymer of a repeating tetrasaccharide unit composed of D-glucuronic acid, D-glucose, D-galactose, L-fucose and O-acetyl (1:1:1:1:1), and has the structure |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|