A point-of-care test of active matrix metalloproteinase-8 predicts triggering receptor expressed on myeloid cells-1 levels in saliva
| Autor: | Räisänen, Ismo T., Heikkinen, Anna Maria, Pakbaznejad Esmaeili, Elmira, Tervahartiala, Taina, Pajukanta, Riitta, Silbereisen, Angelika, Bostanci, Nagihan, Sorsa, Timo |
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| Přispěvatelé: | Department of Oral and Maxillofacial Diseases, HUS Head and Neck Center, University of Helsinki, Staff Services, University Management, Clinicum, Timo Sorsa / Principal Investigator |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
GINGIVAL CREVICULAR FLUID
CUTTING EDGE IDENTIFICATION PERIODONTAL-DISEASE biomarkers matrix metalloproteinases 3126 Surgery anesthesiology intensive care radiology TREM-1 ECTODOMAIN 313 Dentistry ACTIVATION point-of-care testing triggering receptor expressed on myeloid cells-1 INFLAMMATORY RESPONSES LIGAND NEUTROPHIL COLLAGENASE ACTIVITY periodontitis MMP-8 |
| Popis: | Background This cross-sectional study aims to investigate if a point-of-care (PoC) test of active matrix metalloproteinase-8 (aMMP-8) predicts levels of inflammation amplifier triggering receptor expressed on myeloid cells-1 (TREM-1) and its putative ligand the neutrophil peptidoglycan recognition protein 1 (PGLYRP1) in saliva. Methods Forty-seven adolescents, aged 15 to 17 years, were tested with aMMP-8 PoC test, which was followed by a full-mouth clinical examination of the assessment of periodontal, mucosal, and oral health. TREM-1 and PGLYRP1 levels were analyzed by ELISA. The immunofluorometric assay (IFMA) specific for aMMP-8 was used as the reference method. Results Fourteen saliva samples out of a total of 47 showed positivity for aMMP-8 PoC test. Both the TREM-1 and the aMMP-8 (IFMA) levels were significantly elevated among the aMMP-8 PoC test positives compared with the PoC test negatives (P = 4 mm was significantly lower among the adolescents that had a negative aMMP-8 PoC test result, and TREM-1 levels = 4 mm (P |
| Databáze: | OpenAIRE |
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