Decidual glycodelin-A polarizes human monocytes into a decidual macrophage-like phenotype through Siglec-7
| Autor: | Vijayan, Madhavi, Lee, Cheuk-Lun, Wong, Vera H. H., Wang, Xia, Bai, Kungfeng, Wu, Jian, Koistinen, Hannu, Seppälä, Markku, Lee, Kai-Fai, Yeung, William S. B., Ng, Ernest H. Y., Chiu, Philip C. N. |
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| Přispěvatelé: | Department of Clinical Chemistry and Hematology, HUS Abdominal Center, Department of Obstetrics and Gynecology, University of Helsinki, Helsinki University Hospital Area, HUSLAB, Department of Diagnostics and Therapeutics, HUS Gynecology and Obstetrics |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
FETAL-MATERNAL INTERFACE
BLOOD Macrophage GLYCOSYLATION Trophoblast PROTEIN PAEP DIFFERENTIATION PREGNANCY Glycodelin Siglec embryonic structures Progestagen-associated endometrial protein CELLS Decidua 1182 Biochemistry cell and molecular biology INVASIVENESS Endocrine INDOLEAMINE 2 3-DIOXYGENASE GENE-EXPRESSION |
| Popis: | Decidual macrophages constitute 20-30% of the total leukocytes in the uterus of pregnant women, regulating the maternal immune tolerance and placenta development. Abnormal number or activities of decidual macrophages (dMs) are associated with fetal loss and pregnancy complications, such as preeclampsia. Monocytes differentiate into dMs in a decidua-specific microenvironment. Despite their important roles in pregnancy, the exact factors that regulate the differentiation into dMs remain unclear. Glycodelin-A (PAEP, hereafter referred to as GdA) is a glycoprotein that is abundantly present in the decidua, and plays an important role in fetomaternal defense and placental development. It modulates the differentiation and activity of several immune cell types residing in the decidua. In this study, we demonstrated that GdA induces the differentiation of human monocytes into dM-like phenotypes in terms of transcriptome, cell surface marker expression, secretome, and regulation of trophoblast and endothelial cell functions. We found that Sialic acid-binding Ig-like lectin 7 (Siglec-7) mediates the binding and biological actions of GdA in a sialic acid-dependent manner. We, therefore, suggest that GdA, induces the polarization of monocytes into dMs to regulate fetomatemal tolerance and placental development. |
| Databáze: | OpenAIRE |
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