| Autor: |
Mohamed, Fatima, Neveu, Cédric |
| Přispěvatelé: |
Issouf, Mohamed |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Abstract book-27. WAAVP . 2019; 27. Conference of the World Association for the Advancement of Veterinary Parasitology, Madison, USA, 2019-07-07-2019-07-11, 198 |
| Popis: |
Recent studies have described the involvement of Caenorhabditis elegans p-glycoprotein-2 in lysosome formation and lipid storage. In that respect, we are carefully trying to identify in Heligmosomoides polygyrus bakeri the free-living nematode model Caenorhabtidis elegans p-glycoprotein homologs using degenerate primers. These experiments have allowed the identification of five partial p-glycoprotein sequences. Among those Hba-pgp-2 has particular interest. Since, C. elegans PGP-2 is involved in the transport of cholesterol. Cholesterol is needed for molting and growth of the nematodes. In this study, a full-length cDNA sequence corresponding to Hba-pgp-2 was obtained. It presents high homologies with the Cel-pgp-2 gene sequence. Moreover, we showed that Hba-pgp-2 is expressed among the eggs, fourth-stage larvae and adult and their expression could be induced by cholesterol. In order to characterize the Hba-pgp-2 function, the Hba-pgp-2 promoter was analyzed using the nematode model C. elegans and complementation of C. elegans pgp-2 mutant by Hba-pgp-2 was performed. This opens the way for studying the involvement of Pgps in parasitic biological process that can lead to develop new way to control nematode parasites. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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