| Popis: |
The microcirculation is the site of oxygen exchange in the body, and little work has been done to determine if different microvascular beds respond similarly to a simultaneous vascular challenge. A hybrid microvascular monitoring device was developed that uses hyperspectral near-infrared spectroscopy and diffuse correlation spectroscopy to simultaneously monitor the brain and skeletal muscle. Experiments were conducted on Sprague Dawley rats (n=6, 156g±6.4g) to discern the effect that phenylephrine (0.1 mL bolus, 10 mg/kg) has on the mean arterial pressure (MAP), hemoglobin concentration, and blood flow in each microvasculature. Hemoglobin concentration increased by 2.1±0.2 mmol in the brain, and decreased by 2.5±1.0 mmol in skeletal muscle. Cerebral blood flow increased by 20.8±7.2% while muscular blood flow decreased by 7.5±1.5%. The response in the brain was dependent on the initial baseline MAP. This non-invasive method can be used to aid in clinical translation of findings in animal models. |
