| Autor: |
Souza Gil, Eric de, Rezende, Stefani Garcia, Júnior, Eli José Miranda Ribeiro, Barcelos, Hernane Toledo, Scalize, Paulo Sérgio, Santiago, Mariangela Fontes, Quintino, Michelle Pereira, Somerset, Vernon Sydwill |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Popis: |
Laccase is a poliphenoloxidase enzyme that catalyzes the oxidation of phenolic compounds in the corresponding quinones. The current obtained in this redox process can be used for quantitative analysis. In this work, a carbon paste biosensor modified gluteraldehyde functionalized silica and an enzymatic extract of the Pycnoporus sanguineus fungi as a lacase source is proposed for phenol determination. The effect of carbon paste and electrolyte composition, pH from 3.0 to 8.0, start potential from 0.55 to 0.25 mV, scan rate from 5 to 25 mV s-1 and potential pulse amplitude from 10 to 60 mV on the differential pulse voltammetric response was investigated. A linear correlation of r² = 0.9946 was obtained for the phenol content (catechol) in the concentration range from 50 to 500 nmol L-1, with a detection limit of 30 nmol L-1. This biosensor was used for the determination of different kinds of phenolic compounds, presenting a better response for catechol. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
