Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania
| Autor: | Mugasa, C. M., Deborggraeve, S., Schoone, G. J., Laurent, T., Leeflang, M. M., Ekangu, R. A., El Safi, S., Saad, A. F., Basiye, F. L., De Doncker, S., Lubega, G. W., Kager, P. A., Büscher, P., Schallig, H. D. |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Leishmania
Glossina Tsetse flies Laboratory techniques and procedures Protozoal diseases Vectors Oligochromatography NASBA Reproducibility Polymerase chain reaction Assays Trypanosomiasis African PCR Sandflies parasitic diseases Diagnosis Dipstick Trypanosoma brucei Repeatability Leishmaniasis Molecular diagnostic techniques |
| DOI: | 10.1111/j.1365-3156.2010.02547.x |
| Popis: | Summary Objective To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories. Methods The tests are based on PCR or NASBA amplification of the parasites nucleic acids followed by rapid read-out by oligochromatographic dipstick (PCR-OC and NASBA-OC). Results On purified nucleic acid specimens, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 91.7% and 95.5%; Tryp-NASBA-OC, 95.8% and 100%; Leish-PCR-OC, 95.9% and 98.1%; Leish-NASBA-OC, 92.3% and 98.2%. On blood specimens spiked with parasites, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 78.4% and 86.6%; Tryp-NASBA-OC, 81.5% and 89.0%; Leish-PCR-OC, 87.1% and 91.7%; Leish-NASBA-OC, 74.8% and 86.2%. Conclusion As repeatability and reproducibility of the tests were satisfactory, further phase II and III evaluations in clinical and population specimens from disease endemic countries are justified |
| Databáze: | OpenAIRE |
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