Comparison of a commercially available rep-PCR system (Diversilab(R)) with PCR-ribotyping for typing of Clostridium difficile strains

Autor:	Eckert, C, Van Broeck, Johan, Spigaglia, P, Burghoffer, B, Delmée, Michel, Mastrantonio, P, Barbut, F
Přispěvatelé:	Université Pierre et Marie Curie, Paris, France - ER8, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, Istituto Superiore di Sanità, Rome, Italy - Department of Infectious, Parasitic and Immune-mediated Diseases, UCL - (SLuc) Service de microbiologie
Jazyk:	angličtina
Rok vydání:	2011
Předmět:	Molecular Typing Adult Clostridium viruses PCR-Ribotyping bacteria Humans Clostridium difficile biochemical phenomena metabolism and nutrition Sensitivity and Specificity Polymerase Chain Reaction
Zdroj:	Journal of Clinical Microbiology, Vol. 49, no. 9, p. 3352-3354 (2011)
Popis:	This study compared a rep-PCR method (DiversiLab® system) to PCR-ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR-ribotype 027, different rep-types could be distinguished. Rep-PCR showed a higher discriminatory power than PCR-ribotyping. Nevertheless, this method requires technical skill and visual interpretation of rep-PCR fingerprint patterns may be difficult.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od______1493::0d219b90798cbc38e2ad566425c86864 https://hdl.handle.net/2078.1/81691 Zobrazit plný text záznamu