Neuralized promotes basal to apical transcytosis of delta in epithelial cells
| Autor: | Benhra, Najate, Vignaux, Françoise, Dussert, Aurore, Schweisguth, François, Le Borgne, Roland |
|---|---|
| Přispěvatelé: | Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Génétique Fonctionnelle de la Souris, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported in part by grants from the Re´gion Bretagne (ACOMB ‘Notasid' 2168), from the ATIP program Centre National de la Recherche Scientifique, Association pour la Recherche sur le Cancer 4905, and Fondation pour la Recherche Médicale., De Villemeur, Hervé |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
MESH: Mutation
MESH: Drosophila Proteins [SDV.BC]Life Sciences [q-bio]/Cellular Biology MESH: Ubiquitin-Protein Ligases MESH: Drosophila melanogaster MESH: Dogs MESH: Epithelial Cells MESH: Endocytosis [SDV.BDD] Life Sciences [q-bio]/Development Biology MESH: Sense Organs MESH: Animals MESH: Membrane Proteins MESH: Pupa MESH: Cell Polarity MESH: Receptors Notch [SDV.BDD]Life Sciences [q-bio]/Development Biology [SDV.BC] Life Sciences [q-bio]/Cellular Biology |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, 2010, 21 (12), pp.2078-86. ⟨10.1091/mbc.E09-11-0926⟩ |
| ISSN: | 1939-4586 |
| DOI: | 10.1091/mbc.E09-11-0926⟩ |
| Popis: | International audience; Notch receptors mediate short-range signaling controlling many developmental decisions in metazoans. Activation of Notch requires the ubiquitin-dependent endocytosis of its ligand Delta. How ligand endocytosis in signal-sending cells regulates receptor activation in juxtaposed signal-receiving cells remains largely unknown. We show here that a pool of Delta localizes at the basolateral membrane of signal-sending sensory organ precursor cells in the dorsal thorax neuroepithelium of Drosophila and that Delta is endocytosed in a Neuralized-dependent manner from this basolateral membrane. This basolateral pool of Delta is segregated from Notch that accumulates apically. Using a compartimentalized antibody uptake assay, we show that murine Delta-like 1 is similarly internalized by mNeuralized2 from the basolateral membrane of polarized Madin-Darby canine kidney cells and that internalized ligands are transcytosed to the apical plasma membrane where mNotch1 accumulates. Thus, endocytosis of Delta by Neuralized relocalizes Delta from the basolateral to the apical membrane domain. We speculate that this Neuralized-dependent transcytosis regulates the signaling activity of Delta by relocalizing Delta from a membrane domain where it cannot interact with Notch to another membrane domain where it can bind and activate Notch. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|