Human Chorionic Gonadotropin Expression in Human Trophoblasts from Early Placenta: Comparative Study Between Villous and Extravillous Trophoblastic Cells.: hCG in invasive extravillous cytotrophoblast

Autor: Handschuh, Karen, Guibourdenche, Jean, Tsatsaris, Vassilis, Guesnon, Mickaël, Laurendeau, Ingrid, Evain-Brion, Danièle, Fournier, Thierry
Přispěvatelé: Développement humain : Croissance et différenciation, Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biochimie foeto placentaire, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hopital Debré, Maternité Port-Royal [CHU Cochin], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire de génétique moléculaire, Université Paris Descartes - Paris 5 (UPD5)-EA3618, This work was supported by 'la Caisse d'Assurance Maladie des Professions Libérales-Province', CAMPLP, Paris la Défense, 92042, France., Evain-Brion, Danièle
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Zdroj: Placenta
Placenta, 2007, 28 (2-3), pp.175-184. ⟨10.1016/j.placenta.2006.01.019⟩
ISSN: 0143-4004
DOI: 10.1016/j.placenta.2006.01.019⟩
Popis: Human trophoblast differentiates into two pathways: extravillous cytotrophoblasts (EVCT) that invade the uterus wall and villous cytotrophoblasts (VCT) that fuse to form the syncytiotrophoblast (ST) involved in placental exchanges and endocrine function. It is established that hCG is produced and secreted by the ST into the maternal compartment where it plays a key endocrine role and stimulates ST formation in an autocrine manner. Herein, we investigated hCG expression in early placentas by immunohistochemistry using different antibodies. We then compared hCG secretion by primary cultures of VCT and EVCT isolated from the same first trimester human chorionic villi. In situ hCG was immunodetected in EVCT all along their invasive differentiating pathway except in cells near the stromal core of the proximal column. hCG expression was confirmed in vitro by immunocytochemistry and hCG secretion quantified in cell supernatants. Interestingly, whereas hCG secretion increased during VCT differentiation into ST (from 60 to 350UI/L/mug DNA), EVCT secretion remained constant and at a high level during the same culture period (160UI/L/mug DNA). Our data demonstrated that in addition to the ST, invasive EVCT also expressed and secreted high levels of hCG, suggesting a specific paracrine and/or autocrine role for hCG from EVCT origin.
Databáze: OpenAIRE
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