Misdiagnosis of imported falciparum malaria from African areas due to an increased prevalence of pfhrp2/pfhrp3 gene deletion: the Djibouti case
| Autor: | Iriart, Xavier, Menard, Sandie, Chauvin, Pamela, Mohamed, Hasna, Charpentier, Elena, Mohamed, Mohamed, Berry, Antoine, Aboubaker, Mohamed |
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| Přispěvatelé: | PINIER, CHRISTINE, Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Parasitologie et Mycologie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Hôpital Général Peltier, Laboratoire d’analyse médicale Mer Rouge |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
MESH: Antigens
Protozoan / genetics MESH: Humans Plasmodium falciparum MESH: Protozoan Proteins / genetics MESH: Djibouti histidine-rich protein 2 rapid diagnostic test MESH: Sensitivity and Specificity MESH: Population Surveillance MESH: Plasmodium falciparum / classification MESH: Gene Deletion [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases parasitic diseases [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: Diagnostic Errors Djibouti deletion MESH: Malaria Falciparum / diagnosis MESH: Plasmodium falciparum / genetics MESH: Prevalence MESH: Diagnostic Tests Routine |
| Zdroj: | Emerging microbes & infections Emerging microbes & infections, 2020, 9 (1), pp.1984-1987. ⟨10.1080/22221751.2020.1815590⟩ |
| ISSN: | 2222-1751 |
| Popis: | International audience; Following the diagnosis of a falciparum malaria case imported from Djibouti and not detected by a pfHRP2-based rapid diagnostic test (RDT), we investigated the prevalence of the pfhrp2/pfhrp3-deleted parasites in Djibouti using 378 blood samples collected between January and May 2019, from Djiboutian patients with suspected malaria. Malaria diagnosis by quantitative PCR confirmed the presence of Plasmodium falciparum for 20.9% (79/378) samples while RDTs did not detect HRP2 antigen in 83.5% (66/79) of these samples. Quantitative PCRs targeting the pfhrp2/pfhrp3 genes confirmed the absence of both genes for 86.5% of P. falciparum strains. The very large number (86.5%) of falciparum parasites lacking the pfhrp2/pfhrp3 genes observed in this study, now justifies the use of non-HRP2 alternative RDTs in Djibouti. In this area and in most countries where HRP2-based RDTs constitute the main arsenal for falciparum malaria diagnosis, it is important to implement a systematic surveillance and to inform biologists and clinicians about the risk of malaria misdiagnosis. Further investigations are needed to better understand the mechanism of selection and diffusion of the pfhrp2/pfhrp3-deleted parasites |
| Databáze: | OpenAIRE |
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