| Autor: |
Low, Wei Ching, Rujitanaroj, Pim-On, Lee, Dong-Keun, Kuang, Jinghao, Messersmith, Phillip B., Chan, Jerry Kok Yen, Chew, Sing Yian |
| Přispěvatelé: |
School of Chemical and Biomedical Engineering, Lee Kong Chian School of Medicine (LKCMedicine) |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Popis: |
In this study, we promote neuronal differentiation of human mesenchymal stem cells (MSCs) through scaffold-mediated sustained release of siRNA targeting RE-1 silencing transcription factor (REST). Poly (ϵ-caprolactone) nanofibers were surface modified with mussel inspired DOPA-melanin (DM) coating for adsorption of REST siRNA. DM modification increased siRNA-loading efficiency and reduced the initial burst release. Fiber alignment and DM modification enhanced REST knockdown efficiencies. Under non-specific differentiation condition, REST silencing and fiber topography enhanced MSC neuronal markers expressions and reduced glial cell commitment. Such scaffolds may find useful applications in enhancing MSCs neuronal differentiation under non-specific conditions such as an in vivo environment. NMRC (Natl Medical Research Council, S’pore) Accepted version |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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