Standardization of Caco-2 cell culture as in vitro model for intestinal permeability

Autor:	Rodríguez, Yanina I., Abalos, Ivana S., Lozano, Verónica, Cereseto, Marina, Silberman, Nicolás G., Chiale, Carlos, Gruñeiro, Elena, Pesce, Guido
Jazyk:	angličtina
Rok vydání:	2011
Předmět:	Técnicas de Cultivo de Célula atenolol biopharmaceutics classification system Caco-2 permeability Permeabilidad Farmacia
Popis:	The aim of this study was to find out the optimal experimental conditions for Caco-2 cell culture (time and density) and permeability assays (diffusion system and drug concentration) in order to study the in vitro drugs permeability as a predictive method for drug absorption across intestinal epithelium. The integrity of the monolayers used in each assay was determined by measuring the transepithelial electrical resistance (TEER) and the permeability of the atenolol-a drug which is transported across the monolayers by the paracellular pathway-. The best working condition was obtained with a cell seeding of 7.104 cells/insert in a vertical difussion chamber. In such context, the monolayers had a TEER higher than 550 Ω.cm2 and the apparent permeability coefficient of atenolol was 0.71 ± 0.19 x 10-6 cm/seg. Colegio de Farmacéuticos de la Provincia de Buenos Aires
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od______1329::1dd78679fe5968db443578afb65ccd7a http://sedici.unlp.edu.ar/handle/10915/8097 Zobrazit plný text záznamu