| Autor: |
Cai, Qing, Wiederkehr, Rodrigo Sergio, Jones, Ben, Majeed, Bivragh, Colle, Frederick, Zhang, Lei, Stakenborg, Tim, Fiorini, Paolo, Lagae, Liesbet, Tsukuda, Masahiko, Matsuno, T, Yamashita, Ishiro |
| Přispěvatelé: |
Jacobson, Stephen |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Popis: |
In this paper, the layout, fabrication and preliminary testing of a microfluidic chip for the quantitative detection of microRNA (miRNA) molecules is described. The chip integrates the following steps for miRNA quantification: miRNA release, reverse transcription and digital droplet PCR. The experiments for quantifying let-7a miRNA copy numbers were performed using MCF-7 cells. Results show that the digital on-chip and analog off-chip quantifications are in good agreement; the determined concentration on the same sample was 780 templates/L and 900 template/l, respectively. ispartof: pages:1015-1017 ispartof: Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences pages:1015-1017 ispartof: International Conference on Miniaturized Systems for Chemistry and Life Sciences location:San Antonio, TX USA date:26 Oct - 30 Oct 2014 status: published |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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