Analyzing protein clusters on the plasma membrane: application of spatial statistical analyses on super-resolution microscopy images
| Autor: | Paparelli, Laura, Corthout, Nikky, Pavie, Benjamin, Annaert, Wim, Munck, Sebastian |
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| Přispěvatelé: | DeVos, WH, Munck, S, Timmermans, JP |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
PATTERNED EXCITATION MICROSCOPY
Science & Technology STRUCTURED-ILLUMINATION MICROSCOPY Cell Biology Anatomy & Morphology OPTICAL RECONSTRUCTION MICROSCOPY GPI-ANCHORED PROTEINS T-CELL-ACTIVATION PHOTOACTIVATED LOCALIZATION MICROSCOPY RESOLUTION FLUORESCENCE MICROSCOPY FLUID-MOSAIC MODEL GROUND-STATE-DEPLETION Life Sciences & Biomedicine PAIR-CORRELATION-ANALYSIS |
| Popis: | The spatial distribution of proteins within the cell affects their capability to interact with other molecules and directly influences cellular processes and signaling. At the plasma membrane, multiple factors drive protein compartmentalization into specialized functional domains, leading to the formation of clusters in which intermolecule interactions are facilitated. Therefore, quantifying protein distributions is a necessity for understanding their regulation and function. The recent advent of super-resolution microscopy has opened up the possibility of imaging protein distributions at the nanometer scale. In parallel, new spatial analysis methods have been developed to quantify distribution patterns in super-resolution images. In this chapter, we provide an overview of super-resolution microscopy and summarize the factors influencing protein arrangements on the plasma membrane. Finally, we highlight methods for analyzing clusterization of plasma membrane proteins, including examples of their applications. ispartof: Advances in Anatomy, Embryology and Cell Biology vol:219 pages:95-122 ispartof: location:Germany status: published |
| Databáze: | OpenAIRE |
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