Searching for biomarkers in extreme environments using advanced analytical pyrolysis techniques
| Autor: | González-Pérez, José Antonio, Rosa Arranz, José M. de la, Jiménez Morillo, N. T., San Emeterio, Layla M. |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Portuguese Foundation for Science and Technology (FCT) |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Popis: | Comunicación oral presentada en el 1st European Meeting on Geomicrobiology of volcanic caves. días 2-3 de marzo de 2023 celebrado en la Casa de la Ciencia-CSIC de Sevilla Pyrolysis (Py) is the thermochemical decomposition of organic materials (OM) at elevated temperatures without oxygen. The products of Py are amenable to chromatographic separation which, when combined with appropriate detectors, yields valid fingerprint information about the composition and molecular structure of complex mixtures of natural and synthetic macromolecules [1-2]. Analytical Py poses several advantages over other techniques for OM characterization: minimal or no sample preparation needed, the small sample size required, and the ability to detect and characterize a wide variety of organic materials in a single analysis, including recalcitrant and low solubility organic forms [3]. Therefore, this technique is convenient for relatively rapid analyses of scarce or precious organic samples such as those that may be found in extreme environments. In this communication, apart from the conventional Py techniques such as Py associated with gas chromatography and mass spectrometry (Py-GC/MS), will give examples of the application of other more advanced variants to speleothems analyses that provide complementary or more accurate information. These include pyrolysis associated with compound-specific isotope analysis (Py-CSIA) and ultra-high resolution analytical Py (Py-GC/Q-TOF) that are unique and available in our labs at IRNAS-CSIC MOSS Group. References: [1] González-Pérez, J.A., et al., Journal of Chromatography A 1388 (2015) 236–243. [2] San-Emeterio, L.M., et al., Applied Science 11 (2021) 6684. [3] Quénéa, K., et al., Journal of Analytical and Applied Pyrolysis 76 (2006) 271–279. We would like to thank the Spanish Ministry of Science and Innovation from the Spanish State Agency (AEI) for funding the projects TUBOLAN (PID2019-108672RJ-I00) and EQC2019-005772-P and the Portuguese FCT (Fundação para a Ciência e a Tecnologia) for the MICROCENO project (PTDC/CTA-AMB/0608/2020). L. M. San Emeterio thanks FPI research grant (BES-2017-07968). D. Monis, A.M. Carmona, and E. Gutiérrez, are acknowledged for technical assistance. |
| Databáze: | OpenAIRE |
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