Characterization of resistance to CMV Subgroup I strains in Introgression Lines (IL) containing two and three Quantitative Trait Loci (QTL) in melon
| Autor: | Areco, Lorena, Martín-Hernández, Ana Montserrat |
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| Rok vydání: | 2022 |
| Popis: | Póster presentado al XX Congreso Nacional de Fitopatología celebrado en Valencia entre el 24 y el 26 de octubre de 2022. Cucumber mosaic virus (CMV) can infect up to 1200 plant species including those with high economic importance such as Solanaceae and Cucurbitaceae families. The Korean melon accession “Songwhan Charmi” (SC)” is one of the CMV-resistant accessions identified to date, carrying an oligogenic and quantitative resistance. A Doubled Haploid Line (DHL) and a Introgression Lines (ILs) populations were developed from a cross between SC and Piel de Sapo (PS). A Quantitative Trait Loci (QTL) analysis in the DHL population revealed a major QTL, cmv1, located in the chromosome XII and conferring total resistance to the subgroup II strain LS but not to the subgroup I strains. cmv1 itself is able to restrict CMV-LS in the bundle sheath (BS) cells. Two additional QTLs, cmvqw3.1, mapping in the chromosome III, and cmvqw10.1, mapping in the chromosome X, are needed in coordination with cmv1 for resistance to SG I strains. In the present work we have developed ILs containing either two or three QTL. These are an IL with cmvqw3.1 and cmv1, an IL carrying cmvqw10.1 and cmv1 and an IL carrying the three QTL. These ILs do not stop CMV-FNY, but delay phloem entry in different extension. However, the ILs with two QTLs are susceptible to CMV-M6, whereas the IL carrying three QTL can stop CMV-M6 infection. Here we aim to characterize the resistance/susceptibility of these ILs to CMV-M6 by quantifying the virus present in the phloem of inoculated leaves. |
| Databáze: | OpenAIRE |
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