Immune and physiological responses of clams (Polititapes rhomboides) under sediment mobilization and seawater warming conditions
| Autor: | Costa, M. M., Gestal, C., Babarro, José M. F., Gilcoto, Miguel, Villacieros-Robineau, Nicolás, Comeau, Luc A., Darriba, Susana, Silva, Elsa, Nieto-Leirós, L., Feio, Hugo M., Pérez, Jaime, Dios, S. |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Popis: | Poster.-- 4th Congress of the International Society of Fish & Shellfish Immunology, December 12-15, 2022, Bode, Norway High-energy hydrodynamic events associated with currents and waves may disturb bivalve mollusks' ecophysiology, especially those buried in the sea bed where the bottom boundary layer dynamics may mobilize and resuspend the surface sediments. Evidence of massive mortality for the clam Polititapes rhomboides (banded carpet shell clam) in 2010 in Galicia (NW Spain) has been associated to warm water temperatures and high wave magnitudes above climatic averages and the presence of rickettsias (intracellular prokaryotic colonies) in gills. To monitor in the laboratory the environmental conditions observed in 2010, clams were subjected to intense sediment mobilization (four cycles of sediment remobilization, each followed by a calm period) and seawater warming (from 15°C to 18°C). Immune system, behavioral, and ecophysiological clams' responses were then evaluated. Nitric oxide (NO) production increased synergistically with seawater warming and sediment remobilization. Taking into account the four cycles of sediment remobilization, clams responded by increasing NO production as early as the first cycle in what we could call an acute effect. However, the most striking effect was the increased NO response after a second stimulation (the following sediment remobilization cycles), suggesting that clams achieve a kind of alertness the first time they are exposed to a stimulus through a mechanism possibly related to "trained immunity". Seawater warming and sediment mobilization presented also synergistic effects causing the lowest valve opening amplitude. Besides, sediment remobilization caused abrupt decrease in clearance rates of clams suggesting that reduced valve opening during this stage may have altered filtration processes of phytoplankton uptake. Metabolic rate as oxygen consumption showed a synergistic increase with both abiotic stressors. Considering the NO response and the ecophysiology data we could suggest that clams respond to stress increasing metabolism to obtain energy (ATP) and oxygen consumption by aerobic respiration. ATP production involves the generation of reactive oxygen species (ROS) including NO as by-products. The anti-oxidant system can balance the ROS production but this could be drastically altered (disruption of cytoskeleton and apoptotic cell death) if ROS production is greater and faster than the antioxidant system can regulate, which seems not to be the case for this particular experiment Project PID2019-106008RB-C21 financed by MCIN/AEl/10.13039/501100011033 |
| Databáze: | OpenAIRE |
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