| Popis: |
In sunflower, the biochemical pathways leading to oil synthesis and accumulation seeds hare been largely stud how these pathways are regulated is less well understood. WRLE KLED1 (WRII) factor, a member of the AP2/ER,EBP class, is considered a kep regulator in the control of carbon allocation between the glycolytic and fatty acid biosynthetic pathways' The AW box (CNTNG(N)7CG) is characterized as an ,4¿WR.I1 ninaing element nith a location-sensitive feature in Arabidopsis. In this work, we identified the sunflowe r WRI1 gen and characterized its activity in electrophoretic mobility shift assays (EMSA). We studied its role as a co-regulator of sunflower genes involved,in plastidial fatty acid synthesis, identifying tbc gene isoforms that are recognized and bound Uy ttre transcription factor. We also determired the base bias at the N positions in the active sunflower AWbox motif. The sunflower AWbox b sequence-sensitive at the nolt-conserved positions, enabling WRll-binding. Moreover, sunflosu WR'I1 could bind to a non-canonical AW-box motif, opening the possibility of searchi¡g fbr ner target genes. |
