Characterization and immune response of electrochemically reduced graphene oxide functionalized with hyaluronic acid on biomedical grade CoCr after tribocorrosion tests

Autor: Sánchez-López, L., Chico, Belén, Ropero de Torres, N., Escudero Rincón, María Lorenza, García-Alonso, M. C., Lozano, R.M.
Rok vydání: 2021
Předmět:
Popis: European Congress EUROCORR2021, 2021 September 20 - 24, virtual event
CoCr alloys are extensively used in biomedical implants for joint replacements due to their excellent wear-corrosion resistance properties and good biocompatibility. Nevertheless, long term prostheses irretrievably undergo wear-corrosion phenomena causing debris and metallic dissolution that can induce adverse cellular reactions such as inflammatory complications, macrophage activation or bone resorption. Several studies can be found in the literature related to biological responses to debris and metallic particles, however, scarce works are addressed towards the biological response of cells contacting CoCr surfaces after tribocorrosion processes. In this study, the in vitro immune response on worn CoCr surfaces after tribocorrosion tests of modified graphenebased compounds on CoCr alloy in simulated physiological solutions (PBS plus 0.3% hyaluronic acid) has been studied. Graphene oxide was electrochemically reduced on CoCr surfaces (CoCrErGO) and further functionalized with hyaluronic acid (CoCrErGOHA). Wear-corrosion tests were carried out by using disks of 38 mm diameter in contact with a cylinder of 7 mm diameter as counterpart (pin). A normal load of 5N was applied on the counterpart at a rotation rate of 120 rpm for 30 Km. Wear-corrosion tests were performed in a phosphate Buffer Saline (PBS) solution with 3 g/L hyaluronic acid. Immune response and inflammatory balance induced by the exposition to the different surfaces modifications of CoCr disks, before and after wear-corrosion assays, was here analyzed in supernatants of mouse macrophage cell cultures (J774A.1).
Databáze: OpenAIRE
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