Plasma proteomics reveals gilthead sea bream (Sparus aurata; Linnaeus, 1758) impairment evoked by Sparicotyle chrysophrii (Van Beneden & Hesse, 1863)
| Autor: | Riera-Ferrer, E., Estensoro, Itziar, Piazzon de Haro, María Carla, Pozo, R. del, Palenzuela, Oswaldo, Sitjà-Bobadilla, Ariadna |
|---|---|
| Rok vydání: | 2022 |
| Popis: | Trabajo presentado en la 15th International Conference of Parasitology, celebrada en Copenhague (Dinamarca) del 21 al 26 de agosto de 2022. [Introduction]: Sparicotylosis is a ubiquitous disease affecting mainly gilthead sea bream (GSB; Sparus aurata) across the Mediterranean, and it is caused by the polyopisthocotylean monogenean gill parasite, Sparicotyle chrysophrii (Microcotylidae). The disease involves severe anaemia and white-gill syndrome. This study aims to elucidate how the parasite modulates the GSB health status throughout the infection. [Methods]: Experimental infections were conducted in a recirculating aquaculture system (RAS), exposing naïve GSB (R) to effluent water from S. chrysophrii-infected GSB. An additional tank with unexposed naïve fish (C), was maintained in parallel, with open water flow disconnected from the RAS. Haematological and infection parameters from sampled C and R fish were registered. Plasma samples grouped into 4 categories (high-H, medium-M, low-L and control-C) according to infection intensities, underwent a proteome analysis. Additional assays were performed to validate the proteome analysis findings. [Results]: The differential analysis of the plasma protein abundance revealed a clear separation into 3 groups (H, M-L and C). A pathway analysis was performed with the differentially quantified proteins, indicating that the parasitic infection mainly affected pathways related to homeostasis, complement system and lipid transport. Twenty-two proteins significantly correlated with the infection intensity, highlighting apolipoproteins, globins, biotinidases and complement C3. Validation assays in blood and plasma confirmed these correlations. [Conclusions]: The anaemic condition of severely S. chrysophrii-infected GSB is accompanied by innate immunity impairment, and altered lipid profile and biotin metabolism, besides the evident hypoxia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|