Lessons from icosahedral and flexuous viral structures of whitefly-transmitted members of the genera Torradovirus and Ipomovirus
| Autor: | Ferriol, Inmaculada, Byrne, Matthew J., Javed, Avid, Ranson, Neil A., Thuenemann, Eva C., Lomonossoff, George P., López-Moya Gómez, Juan José |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Popis: | Trabajo presentado en la III Reunión de la Red Nacional de Virología de Plantas, RENAVIPLANT, celebrada en Murcia entre el 25 y el 27 de mayo de 2022. Virus-like particles (VLPs) have been used to understand the requirements for virion formation and to solve the structure of many plant viruses. For this purpose, plant transient expression systems have been successfully adopted (e.g.: pEAQ- and pEff-system). We explored the possibility of producing VLPs of two whitefly-transmitted viruses: i) tomato apex necrosis virus (ToANV, genus Torradovirus, family Secoviridae), which has icosahedral virions and causes infection in tomato crops; and ii) cucumber vein yellowing virus (CVYV, genus Ipomovirus, family Potyviridae), which has flexuous virions and causes infection in cucurbit crops. The pEAQ-vector system was initially used to produce the capsid protein (CP) of CVYV and the three CPs of ToANV in Nicotiana benthamiana plants. Successful expressions were confirmed by Western Blot (WB) with the corresponding specific anti-CP antibodies. However, with this system we were only able to confirm VLP production for ToANV by transmission electron microscopy (TEM). Thus, for CVYV we explored the possibility of using the replicative vector, pEff, which allows assembly of the CP on replicating RNA. The system allowed accumulation of CVYV CP, confirmed by WB, and the production of VLPs, confirmed by TEM. In addition, a high-resolution structure of ToANV VLPs was determined using cryo-electron microscopy (Cryo-EM) and compared with that of another aphid-transmitted member of the same family, broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae). The novel structural information will be used for biotechnological applications, and to explore the mechanisms of transmission of these three viruses. |
| Databáze: | OpenAIRE |
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