Yeast extracellular vesicles and whole cells induce similar transcriptomic responses by target wine yeast cells

Autor: Mejías-Ortiz, Miguel, Mencher-Beltrán, Ana, Tronchoni, Jordi, González García, Ramón, Morales, Pilar
Rok vydání: 2022
Popis: Trabajo presentado en la Copenhagen Bioscience Conferences, celebrado en Copenhague (Dinamarca), del 8 al 12 de mayo de 2022
During the last two decades, the use of alternative yeast species (non-Saccharomyces) with different technological or quality-related purposes has been gaining popularity in the wine industry. To ensure complete fermentations, they are most often used in combination with Saccharomyces cerevisiae. This results in potential interspecies interactions that might have an impact on the outcome of wine fermentation. In previous works, our group had shown that short term contact between S. cerevisiae and other wine yeast species, in synthetic grape must, resulted in transcriptional responses with both common and species-specific features. Extracellular vesicles (EVs) have been hypothesised to play a role in these biological interactions. This hypothesis was tested in this work by challenging Saccharomyces cerevisiae cultures with purified Metschnikowia pulcherrima EVs. The impact of EVs on the physiology of S. cerevisiae was evaluated by transcriptomic analysis and compared to the response induced by M. pulcherrima whole cells under the same experimental conditions. This analysis showed an important overlapping in the transcriptional responses induced in S. cerevisiae by either M. pulcherrima whole cells or EVs purified by ultracentrifugation. Among other pathways, both seem to induce transcription of genes related to glycolysis and ribosomal activity; and to repress vacuolar transport. These results confirm a direct response of S. cerevisiae to competing species under wine-like conditions. This is also the first experimental support to the hypothesis that the recognition mechanisms involve, at least in part, EVs.
Databáze: OpenAIRE
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