The microbial volatile-responsive redox-sensitive CYS154 residue of the Calvin-Benson enzyme fructose-1,6-bisphosphate1 is an important determinant of photosynthetic activity in Arabidopsis
| Autor: | Gámez-Arcas, Samuel, Muñoz Pérez, Francisco José, Serrato, Antonio J., Sánchez-López, Ángela María, Baroja-Fernández, Edurne, Bahaji, Abdellatif, Almagro, Goizeder, Leal-López- Jesús, León Morcillo, Rafael Jorge, Pozueta Romero, Javier |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Popis: | Resumen del trabajo presentado en el XVI Meeting Of Plant Molecular Biology (XVI RBMP), celebrado en Sevilla (España), del 14 al 16 de septiembre de 2022 Microorganisms emit volatile compounds (VCs) that promote plant growth and photosynthesis as well as strong developmental and metabolic changes through multiple and complex regulatory mechanisms including global reduction of the thiol redox proteome (Gámez-Arcas et al. 2022). Recently, we found that microbial VC treatment promotes the reduction of the Cys₁₅₄ residue of the redox-regulated Calvin-Benson cycle enzyme fructose-1,6-bisphosphatase 1 (cFBP1) (Ameztoy et al. 2019). Although highly conserved throughout land plants and algae, this residue is not located in the proposed regulatory Trx redox or catalytic domains of cFBP1. To investigate the role played by Cys¹⁵⁴ in the activity of cFBP1 and the response of plants to microbial VCs, we produced and characterized recombinantly produced wild type (WT) cFBP1 (cFBP1wt) and a mutated form of cFBP1 in which the Cys₁₅₄ residue has been replaced by serine (cFBP1mut). We also produced and characterized cfbp1 plants ectopically expressing cFBP1wt and cFBP1mut under the control of the cFBP1 promoter. In native gels, the electrophoretic mobilities of recombinantly produced cFBP1 and cFBP1mut were different. In addition, recombinant cFBP1mut had ca. 85% less activity than cFBP1wt. The ectopic expression of cFBP1wt, and to a lesser extent that of cFBP1mut, countered the reduced photosynthetic activity of cFBP1-lacking cfbp1 plants, reverting it to the WT. Results presented in this work provide strong evidence that the Cys₁₅₄ residue of cFBP1 is an important determinant of photosynthetic activity in Arabidopsis. This work was supported by the Ministerio de Ciencia e Innovación (MCIN) and Agencia Estatal de Investigación (AEI) / 10.13039/501100011033/ (grants BIO2016-78747-P, PID2019-104685GB-100 and PGC2018-096851-B-C21). |
| Databáze: | OpenAIRE |
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