Bioprospection of new compounds in pristine environments produced by bacterial isolates

Autor: Gatinho, Patrícia, Salvador, Catía, Macedo Arantes, Silvia, Martins, M. Rosario, Miller, A. Z.
Přispěvatelé: Fundação para a Ciência e a Tecnologia (Portugal), Ministerio de Ciencia e Innovación (España)
Jazyk: angličtina
Rok vydání: 2023
ISSN: 1462-2920
Popis: Comunicación oral presentada en el 1st European Meeting on Geomicrobiology of volcanic caves. días 2-3 de marzo de 2023 celebrado en la Casa de la Ciencia-CSIC de Sevilla
Pristine environments can be defined as places with limited or no connections to anthropogenic activities [1], for example karstic and marine caves, relevant locations of Natural and Cultural Heritage. Usually, these environments are exposed to extreme factors such as, temperature, salinity, osmolarity, UV radiation, pressure, or pH, with values close to the limit of life. In these extreme environments living organisms biosynthesize secondary metabolites with potential bioactivities giving them unique survival skills to grow in hostile conditions [2]. This study is being performed within the MICROCENO and TUBOLAN research projects and aims to search for new bioactive compounds produced by bacterial cultures, isolated from volcanic caves. Samples were collected from caves of Selvagens Islands (Madeira archipelago, Portugal) and Lanzarote Island (Canary archipelago, Spain). Cultivable microorganisms were previously isolated on different culture medium and identified by sequencing 16S rDNA [3]. The antioxidant activity and antimicrobial spectra against Gram-negative and Gram-positive bacteria was evaluated. The results obtained suggest that selected bacteria isolates produce biologically active compounds with potential application in biotechnology and biomedicine. Additionally, screening assays testing antitumor potential of supernatants from bacterial strain cultures against a breast cancer epithelial cell line MDA-MB-231 and colorectal adenocarcinoma cell line Caco-2 are in progress. Bioprospection and discovery of new compounds represent an opportunity for implement new products obtained by fast and low-cost biotechnological processes as a novel greensafe and sustainable solutions. References: [1] S. Atashgahi, MM. Häggblom, H. Smidt. Environmental Microbiology 20(3), 2018, 934–48. https://doi.org/10.1111/1462-2920.14016. [2] D. Giordano, Marine drugs of MDPI 19(11), 2020; 1–7. https://doi.org/10.3390/md19110642. [3] A. T. Caldeira, N. Schiavon, G. Mauran,C. Salvador,T. Rosado, J. Mirão, A. Candeias. Coatings of MDPI 11(2),2021;1–17.
Funder of Foundation for Science and Technology, I.P., within the scope of the projects UIDB/04449/2020, MICROCENO (PTDC/CTA-AMB/0608/2020), ART3mis (2022.07303.PTDC) and C. Salvador (DL 57/2016/CP1372/CT0019) to individual support. The Spanish Ministry of Science and Innovation is also acknowledged for funding the TUBOLAN project (PID2019-108672RJ-I00).
Databáze: OpenAIRE
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