Progress in the analysis of English Channel loliginid squid diets using DNA-barcoding techniques
| Autor: | Larivain, A., Zatylny-Gaudin, Céline, Pierce, Graham J., Power, Annie Marie, Robin, Jean-Paul |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Popis: | Cephalopod International Advisory Council Conference, Cephalopods in the Anthropocene: Multiple Challenges in a Changing Ocean, April 2-8, 2022, Sesimbra, Portugal Study of the diet of squid is hampered by the fact that these animals manipulate their prey and do not necessarily ingest the hard parts that allow the determination of prey remains present in the stomach. The result is a large percentagen of "indeterminate teleost fish" in stomach contents. This is widely described in the literature and has also been found in samples from the English Channel of the two loliginid squid exploited in this area (Loligo forbesii and Loligo vulgaris). New biological samples of both species were collected at the Port-en-Bessin fish market during the 2019-2020 fishing season. Stomach content DNA was extracted and the COI gene was selected by PCR and sequenced using a highthroughput next generation sequencing. The DNA barcoding results were compared with the databases available online. These preliminary results validate a protocol based on commercial samples kept frozen before analysis. Sequences allowed 34 different types of prey to be determined to the species level, including 17 teleost fish species. Although cannibalism could not be proven, accurate determination of the remains of cephalopod prey underlined the high occurrence of cuttlefish eaten by both species of squid. Food composition was analysed in order to make comparisons between species, between seasons (in L. vulgaris) and according to the predator size |
| Databáze: | OpenAIRE |
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