Identification of microbial biomarkers for fish mucosal health
| Autor: | Toxqui-Rodríguez, S., Sitjà-Bobadilla, Ariadna, Piazzon de Haro, María Carla, Pérez-Sánchez, Jaume |
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| Rok vydání: | 2022 |
| Popis: | Trabajo presentado en el 22nd Fish Immunology, celebrado en Wageningen (Países Bajos) del 24 al 28 de abril de 2022. Mucosal (gut, skin, gills) microbial patterns will be assessed in farmed gilthead sea bream (Sparus aurata), combining the use of conventional (Illumina platform) and portable (Oxford Nanopore, MinION) platforms for bacteria sequencing and taxonomic assignment of short- (v3-v4) and long-reads (v1-v9) of 16S rRNA gene, respectively. Changes in microbial patterns in response to biotic (host genetics, pathogen exposure) and abiotic (water temperature, oxygen availability, diet) factors will be assessed to relate changes in the mucosal metagenome and metatranscriptome with a healthy and stress resilience condition. Such data in combination with host transcriptomics (RNA-seq, targeted PCR-arrays), composition and activation of immune cells in gut and gills, and operational welfare indicators based on indicators of growth performance, external tissue damage, and behavioral traits (data-logger measures, activity, and ventilation rates) will serve to fed a meta-analysis system with machine-learning capacity. This will include the combined use of multivariate discriminant analyses (PLS-DA) and Bayesian networks, which will allow correlating microbial taxa among them and with challenging experimental variables. The combination of background knowledge and the previously mentioned tools will allow monitoring changes in the microbiota of gilthead sea bream in response to biotic and abiotic factors and link them to fish health and performance indicators. Thus, filling fish microbiota knowledge gaps by integrating newly generated information will help understand which microbial taxa and genes relate to host health in aquaculture. |
| Databáze: | OpenAIRE |
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