Sphingolipid interactions in model membrane systems (lipid bilayers) containing cholesterol
| Autor: | González-Ramírez, Emilio J., García Arribas, Aritz B., Sot, Jesús, Goñi, Félix M., Alonso, Alicia |
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| Rok vydání: | 2022 |
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| Popis: | Póster presentado al 8th International Iberian Biophysics Congress celebrado en Bilbao los días 20 y 21 de junio de 2022. Sphingolipid molecular interactions are important because of their structural functions and of their role as second messengers in some cellular processes. Among them, sphingomyelin (SM) is the major sphingolipid component of mammalian cells, in whose plasma membrane it can constitute up to 10 mol% of the phospholipids. SM has attracted the attention of scientists due to its strong interaction with other lipids, such as cholesterol (Chol), which interacts with SM giving rise to cholesterol-enriched domains in a liquid ordered (L0) phase, and ceramide, which segregates into highly packed gel domains that could act as signaling platforms in several cellular processes as apoptosis, cell senescence and death. Recent lipidomic studies quantified the presence of different natural sphingolipids in several mammal tissues, showing that longer and saturated ceramides such as d18:1/18:0 and d18:1/24:0 predominate in brain tissue, while d18:1/16:0 are most abundant in other tissues. To further study the interactions between the different sphingolipids in the plasma membrane, and trying to elucidate how their distribution affects their biophysical properties, a liquid- disordered/liquid-ordered (LdL0) cell membrane environment is recreated in a membrane model system composed of DOPC:SM:Chol (2:1:1) + Cer. To characterize this model, a combination of techniques was used: atomic force microscopy (AFM), confocal microscopy, and differential scanning calorimetry (DSC). |
| Databáze: | OpenAIRE |
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