| Autor: |
Sriskandarajah, Sridevy, Clapham, David, Lundquist, Per-Olof |
| Jazyk: |
švédština |
| Rok vydání: |
2014 |
| Předmět: |
|
| DOI: |
10.4236/ajps.2014.55067 |
| Popis: |
Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing highamounts of several bioactive compounds with nutritional and medicinal traits. It is also planted tocontrol soil erosion. A genetic transformation procedure will facilitate studies of the control ofplant development and interactions with symbionts and pathogens, and will provide a tool forplant breeding. Here, we present a particle bombardment method for transforming seabuckthorn.The early stages of induced adventitious shoots from roots were chosen as a novel target tissue forthe transformation procedure. The root system was bombarded with gold particles coated withplasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression,and shoots were regenerated under kanamycin selection. PCR analysis of the regeneratedtransformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kbfragment from the 35S promoter in three shoots from independent transformation events. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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