Plasma Membrane Sheets for Studies of B Cell Antigen Internalization from Immune Synapses

Autor:	Nowosad, CR, Tolar, P
Přispěvatelé:	European Research Council
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	B cells B cell receptor Plasma membrane sheets Transendocytosis 0601 Biochemistry And Cell Biology Antigen internalization Trogocytosis Cytoskeleton Signaling Immune synapse Developmental Biology
Popis:	Surrogate planar and membrane systems have been employed to study the architecture of immune synapses; however, they often do not recapitulate trans-synaptic extraction and endocytosis of ligands by the immune cells. Transendocytosis (or trogocytosis) of antigen from immune synapses is particularly critical for antigen processing and presentation by B cells. Here we describe a protocol for preparation of plasma membrane sheets (PMSs), which are flexible and fluid membrane substrates that support robust B cell antigen extraction. We show how to attach B cell antigens to the PMSs and how to investigate antigen extraction and endocytosis by fluorescent microscopy and computational image analysis. These techniques should be broadly applicable to studies of transendocytosis in a variety of cellular systems.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od______1032::eb378c101ca834fbe5abfaef4b827c3d http://hdl.handle.net/10044/1/48787 Zobrazit plný text záznamu