Naive B cell output in HIV-infected and HIV-uninfected children
| Autor: | Payne, H, Chain, G, Adams, S, Hunter, P, Luckhurst, N, Gilmour, K, Lewis, J, Babiker, A, Cotton, M, Violari, A, Gibb, D, Callard, R, Klein, N |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
B-Lymphocytes Immunity Cellular bone marrow Infant Newborn HIV Infant 1103 Clinical Sciences HIV Infections DNA Models Theoretical Flow Cytometry KRECs Cohort Studies naive B cell output South Africa Ki-67 Antigen Anti-Retroviral Agents Child Preschool Virology Humans Female Child ART Cell Proliferation |
| Popis: | In this study, we aimed to quantify KREC (kappa-deleting recombination excision circle) levels and naive B cell output in healthy HIV-uninfected children, compared with HIV-infected South African children, before and after starting ART (antiretroviral therapy). Samples were acquired from a Child Wellness Clinic (n = 288 HIV-uninfected South African children, 2 weeks-12 years) and the Children with HIV Early Antiretroviral Therapy (CHER) trial (n = 153 HIV-infected South African children, 7 weeks-8 years). Naive B cell output was estimated using a mathematical model combining KREC levels to reflect B cell emigration into the circulation, flow cytometry measures of naive unswitched B cells to quantify total body naive B cells, and their rates of proliferation using the intracellular marker Ki67. Naive B cell output increases from birth to 1 year, followed by a decline and plateau into late childhood. HIV-infected children on or off ART had higher naive B cell outputs than their uninfected counterparts (p = .01 and p = .04). This is the first study to present reference ranges for measurements of KRECs and naive B cell output in healthy and HIV-infected children. Comparison between HIV-uninfected healthy children and HIV-infected children suggests that HIV may increase naive B cell output. Further work is required to fully understand the mechanisms involved and clinical value of measuring naive B cell output in children. |
| Databáze: | OpenAIRE |
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