Quantitative measurement of L1 HPV16 methylation for the prediction of pre-invasive and invasive cervical disease
| Autor: | Kottaridi, C, Kyrgiou, M, Pouliakis, A, Magkana, M, Aga, E, Spathis, A, Mitra, A, Makris, G, Chrelias, C, Mpakou, V, Paraskevaidis, E, Panayiotides, J, Karakitsos, P |
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| Přispěvatelé: | British Society for Colposcopy and Cervical Pathology, Imperial College Healthcare Charity, Genesis Research Trust |
| Rok vydání: | 2016 |
| Předmět: |
LIQUID-BASED CYTOLOGY
Adult Genotype Immunology cervical intraepithelial neoplasia Microbiology Sensitivity and Specificity Uterine Cervical Diseases Young Adult Humans Prospective Studies CIN DNA METHYLATION Aged RISK Human papillomavirus 16 Science & Technology Greece 16 E6 GENE Papillomavirus Infections Human Papillomavirus WOMEN ENDOMETRIAL LESIONS Oncogene Proteins Viral 11 Medical And Health Sciences Middle Aged 06 Biological Sciences CANCER female genital diseases and pregnancy complications United Kingdom Infectious Diseases pyrosequencing INTRAEPITHELIAL NEOPLASIA DNA Viral PREGNANCY OUTCOMES Linear Models Capsid Proteins CpG Islands Female HPV L1 gene methylation Life Sciences & Biomedicine NUCLEAR MORPHOMETRY |
| Popis: | Background: Methylation of the HPV DNA has been proposed as a novel biomarker. Here, we correlated the mean methylation level of 12 CpG sites within L1 gene, to the histological grade of cervical precancer and cancer. We assessed whether HPV L1 gene methylation can predict the presence of high-grade disease at histology in women testing positive for HPV 16 genotype. Methods: Pyrosequencing was used for DNA methylation quantification and 145 women were recruited. Results: We found that the L1 HPV16 mean methylation (+/-SD) significantly increased with disease severity [CIN3=17.9%(±7.2) vs CIN2=11.6%(±6.5), p |
| Databáze: | OpenAIRE |
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