TGF-beta repressors SnoN and Ski are implicated in human colorectal carcinogenesis
| Autor: | Bravou, V, Antonacopoulou, A, Papadaki, H, Floratou, K, Stavropoulos, M, Episkopou, V, Petropoulou, C, Kalofonos, H |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Down-Regulation
Gene Expression C-SKI 0601 Biochemistry and Cell Biology Transforming Growth Factor beta Proto-Oncogene Proteins Pathology PROGNOSTIC MARKER TUMORIGENESIS Humans TUMOR-SUPPRESSOR 1112 Oncology and Carcinogenesis TGF-beta Oncology & Carcinogenesis GENE-EXPRESSION Science & Technology COMPLEX HUMAN CANCER GROWTH-FACTOR-BETA Intracellular Signaling Peptides and Proteins beta-catenin Cell Biology DEGRADATION Ski Colorectal cancer DNA-Binding Proteins Oncology CELLS Disease Progression SnoN Colorectal Neoplasms human activities Life Sciences & Biomedicine Signal Transduction |
| Popis: | Background: The TGF-β signaling repressors SnoN and Ski have been critically implicated in human cancer.Methods: To explore the role of SnoN and Ski in the development and progression of colorectal cancer we examined their protein expression profile by immunohistochemistry in a series of human colorectal adenomas, carcinomas and lymph node metastases. The mRNA expression of SnoN was also quantified by Real-Time RT-PCR.Results: SnoN and Ski were overexpressed both in adenomas with severe dysplasia and colorectal carcinomas. Protein expression was cytoplasmic and nuclear with predominant cytoplasmic localization. The subcellular localization was related differently to pathologic variables of colorectal carcinomas. Although there was no significant association of protein levels with tumor invasion and metastasis, a significant correlation of nuclear SnoN and Ski with β-catenin pathway was observed. Moreover, SnoN mRNA did not differ in carcinomas as compared to normal control and there was no correlation between SnoN protein and mRNA levels.Conclusion: Our findings suggest that SnoN and Ski exert oncogenic effects in human colorectal carcinogenesis and their overexpression is implicated in early stage disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|