Mitochondrial monoamine oxidase-A-mediated hydrogen peroxide generation enhances 5-hydroxytryptamine-induced contraction of rat basilar artery
| Autor: | Chan, SW, Lee, WYW, Lee, SMY, Seto, SW, Leung, GPH, Ho, HP, Au, ALS, Zhang, Q, Li, RWS, Ngai, SM, Poon, CCW, Yeung, JHK, Kwan, YW, Kong, SK |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Basilar artery
Monoamine Oxidase - metabolism Mitochondrial reactive oxygen species Monoamine oxidases Spontaneously hypertensive rats Hypertension - physiopathology Serotonin - administration and dosage - pharmacology cardiovascular system Mitochondria - drug effects - metabolism Hydrogen Peroxide - metabolism 5-hydroxytryptamine |
| Popis: | BACKGROUND AND PURPOSE We evaluated the role(s) of monoamine oxidase (MAO)-mediated H 2O 2 generation on 5-hydroxytryptamine (5-HT)-induced tension development of isolated basilar artery of spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. EXPERIMENTAL APPROACH Basilar artery (endothelium-denuded) was isolated for tension measurement and Western blots. Enzymically dissociated single myocytes from basilar arteries were used for patch-clamp electrophysiological and confocal microscopic studies. KEY RESULTS Under resting tension, 5-HT elicited a concentration-dependent tension development with a greater sensitivity (with unchanged maximum tension development) in SHR compared with WKY (EC 50: 28.4 ± 4.1-nM vs. 98.2 ± 9.4-nM). The exaggerated component of 5-HT-induced tension development in SHR was eradicated by polyethylene glycol-catalase, clorgyline and citalopram whereas exogenously applied H 2O 2 enhanced the 5-HT-elicited tension development in WKY. A greater protein expression of MAO-A was detected in basilar arteries from SHR than in those from WKY. In single myocytes and the entire basilar artery, 5-HT generated (clorgyline-sensitive) a greater amount of H 2O 2 in SHR compared with WKY. Whole-cell iberiotoxin-sensitive Ca 2+-activated K + (BK Ca) amplitude measured in myocytes of SHR was approximately threefold greater than that in WKY (at +60-mV: 7.61 ± 0.89-pA.pF -1 vs. 2.61 ± 0.66-pA.pF -1). In SHR myocytes, 5-HT caused a greater inhibition (clorgyline-, polyethylene glycol-catalase- and reduced glutathione-sensitive) of BK Ca amplitude than in those from WKY. CONCLUSIONS AND IMPLICATIONS 5-HT caused an increased generation of mitochondrial H 2O 2 via MAO-A-mediated 5-HT metabolism, which caused a greater inhibition of BK Ca gating in basilar artery myocytes, leading to exaggerated basilar artery tension development in SHR. © 2010 The Authors. British Journal of Pharmacology © 2010 The British Pharmacological Society. link_to_OA_fulltext |
| Databáze: | OpenAIRE |
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