Jednonukleotidni polimorfizmi gena za β-laktoglobulin, k-kazein i DGAT1 kao kandidati za stroge selekcijske kriterije holštajnskih krava s obzirom na sastav i proizvodnost mlijeka

Autor: Ateya, Ahmed, Nasr, Sherif, Ghanem, Hanaa, Sadek, Kadry, Al-Sharif, Mona, Hendam, Basma
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Veterinarski arhiv
Volume 93
Issue 1
ISSN: 1331-8055
0372-5480
Popis: The aim of this study was to investigate β-Lactoglobulin, k-casein and DGAT1 gene polymorphism and to associate this polymorphism with milk composition and performance traits in Holstein cattle using the PCR-DNA sequencing approach. On the basis of farm records, accurate phenotypic data for milk composition and performance traits were obtained for seventy Holstein dairy cows. Blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR was carried out for amplification of fragments of exon 4 (301-bp) of β-Lactoglobulin, exon 4 (373-bp) of k-casein, and exon 7 (321-bp) of DGAT1 genes. DNA sequencing assessment elaborated single nucleotide polymorphisms (SNPs) in the investigated genes amongst the enrolled dairy cows. On the basis of the dairy cows that harbored identified SNPs in each gene, the animals were allocated into different groups. The least square means of the groups revealed a significant association (P ≤ 0.05) between SNPs and milk production and performance traits. Logistic regression model confirmed a highly significant effect of the identified SNPs on the studied traits, where a moderate to strong relationship was detected between the predictor (SNPs) and the grouping variable (Milk composition and performance traits). Consequently, the identified SNPs in β-Lactoglobulin, k-casein and DGAT1 genes could be used as candidates for developing marker assisted selection (MAS) for milk composition and performance traits in Holstein dairy cattle.
Cilj rada bio je, primjenom PCR-DNA metode i analize sljedova, istražiti polimorfizme gena za β-Lactoglobulin, k-kazein i DGAT1 te procijeniti njihovu povezati sa sastavom mlijeka i svojstvima proizvodnosti goveda holštajnske pasmine. Na temelju evidencija s farmi dobiveni su točni fenotipski podaci o sastavu mlijeka i proizvodnosti 70 muznih krava. Za ekstrakciju DNK prikupljeni su uzorci krvi pojedinačnih krava u epruvete koje su sadržavale dinatrijev EDTA kao antikoagulans. PCR je proveden za amplifikaciju fragmenata egzona 4 (301-bp) β-laktoglobulina, egzona 4 (373-bp) k-kazeina i egzona 7 (321-bp) gena DGAT1. Analiza sljedova DNK prikazala je jednonukleotidne polimorfizme (SNPs) u istraženim genima. Uzevši u obzir krave kod kojih su utvrđeni SNP-ove u svakom genu, životinje su raspoređene u različite skupine. Srednje vrijednosti (LSM) skupina pokazale su znakovitu povezanost (P
Databáze: OpenAIRE
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