Morphological and cytochemical characterization of thrombocytes and leukocytes in hatchlings of three species of Amazonian freshwater turtles

Autor: Adriano T. Oliveira, Wanessa R. Cruz, Jackson Pantoja-Lima, Sihame B. Araújo, Maria L. G. Araújo, Jaydione L. Marcon, Marcos Tavares-Dias
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Veterinarski arhiv
Volume 81
Issue 5
ISSN: 1331-8055
0372-5480
Popis: The aim of this paper was to characterize the morphological and cytochemical aspects of thrombocytes and leukocytes in hatchlings of Podocnemis expansa, P. unifilis and P. sextuberculata from the lower Purus River, Amazonas State, Brazil. Blood smears were submitted to staining by Periodic Acid-Schiff (PAS), Peroxidase (PER), Toluidine Blue (TB), Sudan Black B (SBB) and Bromophenol Blue (BB). Only the lymphocytes did not present a positive-PAS reaction, while heterophils of P. unifilis showed a weak positive reaction. Azurophils, heterophils and eosinophils had a weak positive reaction for PER, with the exception of heterophils in P. expansa which stained intensely. Positive staining with BB was observed in granules of heterophils, eosinophils and basophils of P. expansa, P. unifilis and P. sextuberculata, but this was weak in basophils of P. sextuberculata. Sudanophilia was observed in heterophils granules and in eosinophils of P. expansa, P. unifilis and P. sextuberculata. Metachromasia was demonstrated in basophils of these three species. In these turtle hatchlings, heterophils and eosinophils are the most frequent leukocytes and both have a similar role in defense, since the granules of these granulocytes present glycogen, sudanophilia, peroxidase and basic proteins.
Svrha ovog rada bila je odrediti morfološke i citokemijske značajke trombocita i leukocita mlađa kornjača Podocnemis expansa, P. unifilis i P. sextuberculata iz donjega toka rijeke Purus River u državi Amazona u Brazilu. Razmasci krvi bili su obojeni perjodnom kiselinom i Shiffovim reagensom (PAS), peroksidazom (PER), toluidinskim plavilom (TB), sudanskim crnilom B (SBB) i bromfenolskim plavilom (BB). Samo limfociti se nisu obojili metodom PAS, dok su heterofili vrste P. unifilis pokazivali slabo pozitivnu reakciju. Bazofili, heterofili i eozinofili pokazivali su slabu aktivnost peroksidaze s iznimkom heterofila u vrste P. expansa koji su pokazivali jaču aktivnost. Bromfenolskim plavilom obojila su se zrnca heterofila, eozinofila i bazofila u vrsta P. expansa, P. unifilis i P. sextuberculata, ali je bojenje bilo slabo u bazofifi lima vrste P. sextuberculata. Sudanskim crnilom B obojila su se zrnca heterofila vrsta P. expansa, P. unifilis i P. sextuberculata. Metakromatska zrnca bila su uočena u bazofilima svih triju vrsta. U njihova mlađa najčešće su dokazani heterofili i eozinofili, a i jedni i drugi imaju sličnu ulogu u obrani. Zrnca tih granulocita upućuju na sadržaj glikogena, peroksidaze, bazičnih proteina, a imaju afinitet prema sudanskom bojenju.
Databáze: OpenAIRE
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