Istraživanje primjenjivosti metode detekcije SARS-CoV-2 virusa i njegove pojavnosti u hrani i pakiranju hrane

Autor: Mlinar, Zdenko, Kostelac, Deni, Kovaček, Ivančica, Klobučar, Ana, Tešić, Vanja, Prahin, Vedran, Frece, Jadranka
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Food Technology and Biotechnology
Volume 61
Issue 2
ISSN: 1334-2606
1330-9862
Popis: Research background. While it is clear that SARS CoV-2 coronavirus is the primary respiratory virus, there are no entirely clarified ways of transmission. Foodborne transmission has remained an unexplained path. Therefore, the goals of this paper are to examine and present an assessment of the most appropriate of the four selected kits for RNA extraction for the testing and detection of SARS-CoV-2 on food packaging surfaces, food surfaces, and in food. This will enable to indicate the possibility of infection through contact or direct food consumption. Experimental approach. Finding the best technique is vital as RNA extraction is one of the essential elements in detecting SARS-CoV-2. This was achieved through an experiment with four commercial kits following the original manufacturers’ protocols, and with a modification of the original protocols that included the use of ethanol and isopropanol. The selected kit was used for RNA extraction from the swabs of packaging surfaces, food surface, and ready-to-eat food samples. The coronavirus was then identified using real-time reverse transcription-polymerase chain reaction (RT-PCR) assays to determine whether the SARS-CoV-2 virus or viral particles are present in the food chain with the overall purpose of demonstrating the possibility that food can contribute as a vehicle for the transmission of the virus. Results and conclusions. The findings of this investigation made the most effective extraction kit and protocol stand out. The results of the applicability of the kit indicated a significant share of positive samples of viral SARS-CoV-2 virus particles on surfaces from the environment where infected persons with 'silent' COVID-19 infection, with mild symptoms or no symptoms, were present. However, according to the findings of the second part of the study, the virus was not detected on the examined samples of food packaging surfaces, food surfaces, and food. Novelty and scientific contribution. The presented results distinguished one of the most suitable protocols for isolating RNA from environmental surface samples. The main contribution of the study is in the presentation of the results, that is, the examination of samples that are primarily related to the food chain, food packaging, food surfaces, and ready-to-eat food. The results of this study could also be helpful for further determination of the potential of food as a vector for the transmission of coronaviruses.
Pozadina istraživanja. Iako je jasno da je SARS CoV-2 koronavirus primarno respiratorni virus, nisu u potpunosti razjašnjeni načini njegovog prijenosa. Prijenos hranom je ostao neobjašnjen način prijenosa. Stoga su ciljevi ovoga rada bili ispitati i procijeniti koji je od četiri odabrana komercijalna kompleta za ekstrakciju RNK najprikladniji za testiranje i otkrivanje SARS-CoV-2 na pakiranjima, površini hrane i u hrani. Time bi se mogla utvrditi mogućnost infekcije kontaktom ili izravnom konzumacijom hrane. Eksperimentalni pristup. Pronalaženje najbolje tehnike je ključno, jer je ekstrakcija RNK jedan od bitnih elemenata u otkrivanju SARS-CoV-2. To je postignuto eksperimentom s četiri komercijalna kompleta za ekstrakciju, korištenih u skladu s izvornim protokolima proizvođača i njihovom modifikacijom koja je uključivala upotrebu etanola i izopropanola. Odabrani komplet korišten je za ekstrakciju RNK iz briseva s površina pakiranja, površine hrane i iz uzoraka gotove hrane. Koronavirus je zatim identificiran pomoću testova lančane reakcije polimerazom s reverznom transkripcijom u stvarnom vremenu (real-time RT-PCR), radi utvrđivanja prisutnosti virus SARS-CoV-2 ili virusnih čestica u prehrambenom lancu, sa sveukupnom namjerom dokazivanja mogućnosti prijenosa virusa putem hrane. Rezultati i zaključci. Rezultati ovog istraživanja istaknuli su najučinkovitiji komplet i protokol za izolaciju. Ispitivanjem primjenjivosti kompleta utvrđen je značajan udjel uzoraka pozitivnih na virusne čestice SARS-CoV-2 izdvojenih s površina iz radnog okoliša u kojem su boravile oboljele osobe s 'tihom' infekcijom COVID-19, odnosno s blagim simptomima ili bez simptoma bolesti. Međutim, prema nalazima drugog dijela istraživanja, virus nije detektiran na ispitanim uzorcima pakiranja hrane, površina hrane i hrane. Novina i znanstveni doprinos. Predstavljeni rezultati istaknuli su jedan od najprikladnijih protokola za izolaciju RNK iz okolišnih uzoraka površina. Glavni doprinos studije je u prikazu rezultata, odnosno ispitivanju uzoraka koji se primarno odnose na prehrambeni lanac, ambalažu hrane, površine hrane i gotovu hranu. Rezultati ove studije također bi mogli pomoći daljnjem određivanju potencijala hrane kao vektora za prijenos koronavirusa.
Databáze: OpenAIRE
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