Toksikokinetika pojedinačne intraperitonealne i oralne doze praletrina u miša

Autor: Bhoye Sow, Mamadou, Eraslan, Gökhan
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Veterinarski arhiv
Volume 92
Issue 5
ISSN: 1331-8055
0372-5480
Popis: The aim of this study was to determine the toxicokinetic profile of orally and intraperitoneally administered prallethrin, which is frequently used as an environmental health drug. For this purpose, prallethrin was administered to 42 female 2-3-month-old BALB/c mice, each weighing 35-40 grams, at a dose of 2 mg/kg bw by intraperitoneal and oral route. The mice were randomly assigned to two groups, each of 21 animals. While Group 1 was administered 2 mg/kg bw prallethrin in dimethyl sulfoxide by intraperitoneal route, Group 2 received a single oral dose of 2 mg/ kg bw prallethrin in dimethyl sulfoxide. After the intraperitoneal and oral administration of prallethrin, intra-cardiac blood was drawn into heparinized tubes at certain periods. Plasma prallethrin concentrations were measured by gas chromatography using a micro-electron capture detector. The distribution profile was found to be consistent with the two-compartment open model. The plasma maximum concentration (Cmax), time to reach maximum concentration (tmax), half-life (t1/2β), mean residence time (MRT), area under the curve (AUC0→∞) and bioavailability (F) values for orally administered prallethrin were 3.66±0.78 ng/ml, 0.60±0.05 h, 10.20±1.24 h, 11.72±1.51 h, 15.19±4.43 ng/h. ml and 39.86%, respectively. For intraperitoneally administered prallethrin, the t1/2β, MRT and AUC0→∞ values were calculated as 7.46±0.54 h, 8.05±0.64 h and 38.10±5.80 ng/h.ml, respectively. The data obtained indicates that the oral bioavailability of prallethrin is lower than that of many other pyrethroids such as flumethrin and cypermethrin. Compared to some other pesticides in the same group, such as phenothrin, the half-life and mean residence time of prallethrin in the body are not short, when administered by both routes. The results obtained suggest that exposure to high doses of prallethrin may pose a risk of poisoning. In view of the toxicokinetic parameters determined, this study points out to the need for further studies to better understand the toxicity of prallethrin in mammals.
Cilj je ovog istraživanja bio odrediti toksikokinetički profil oralne i intraperitonealne primjene praletrina, lijeka koji se često upotrebljava jer nije štetan za okoliš. S tom je svrhom praletrin primijenjen u 42 ženke BALB/c miševa stare 2 – 3 mjeseca, tjelesne mase 35 – 40 grams, u dozi od 2 mg/kg intraperitonealno i oralno. Miševi su nasumično podijeljeni u dvije skupine sa po 21 jedinkom u svakoj skupini. Dok je u skupini 1 praletrin primijenjen u dozi od 2 mg/kg u dimetil-sulfoksidu intraperitonealno, životinje u skupini 2 primile su pojedinačnu dozu od 2 mg/kg praletrina u dimetil-sulfoksidu oralno. Nakon intraperitonealne i oralne primjene praletrina, intrakardijalna krv uzeta je u heparinizirane epruvete u određenim intervalima. Koncentracije plazmatskog praletrina izmjerene su plinskom kromatografijom upotrebom mikroelektronskog detektora. Za oralno primijenjen praletrin maksimalna koncentracija u plazmi (Cmax) bila je 3,66 ± 0,78 ng/mL, vrijeme do postizanja maksimalne koncentracije (tmax) 0,60 ± 0,05 h, poluvijek (t1/2β) 10.20±1.24 h, srednje vrijeme zadržavanja (MRT) 11,72 ± 1,51 h, površina ispod krivulje (AUC0→∞) 15,19 ± 4,43 ng/h.mL i bioraspoloživost (F) 39,86 %. Za intraperitonealno primijenjen praletrin vrijednost t1/2β bila je 7,46 ± 0,54 h, MRT-a 8,05 ± 0,64 h i AUC0→∞ 38,10 ± 5,80 ng/h.mL. Rezultati pokazuju da je oralna bioraspoloživost praletrina manja nego kod mnogih drugih piretroida, poput flumetrina i cipermetrina. U usporedbi s drugim pesticidima u istoj skupini, poput fenotrina, poluvijek i srednje vrijeme zadržavanja praletrina u organizmu nisu kratki ako se primjenjuju na oba načina. Rezultati također pokazuju i da je izloženost visokim dozama praletrina rizična zbog mogućeg otrovanja. S obzirom na ustanovljene toksikokinetičke pokazatelje, ovaj rad pokazuje da su potrebna daljnja istraživanja radi boljeg razumijevanja toksičnosti praletrina u sisavaca.
Databáze: OpenAIRE
Externí odkaz: