Quantitative study on the rat parotid gland after orchiectomy

Autor: Davor Ježek, Ljerka Banek, Ružica Pezerović-Panijan, Džemal Pezerović
Jazyk: angličtina
Rok vydání: 1999
Předmět:
Zdroj: Veterinarski arhiv
Volume 69
Issue 1
ISSN: 1331-8055
0372-5480
Popis: In this study the reaction of the rat parotid acini, duct system and the glandular connective tissue was analysed at various times after orchiectomy. Mature Fisher rats were sacrificed at 12 hours and at 3, 8, 15, 30 and 60 days after orchiectomy. Following the removal of parotid glands, histological sections were made and stereologically analysed by Weibel's multipurpose test system. When compared to controls, the volume of the parotid acini (per mm3 of the tissue) in orchiectomized rats was significantly decreased, whereas the volume of the connective tissue (per mm3 of the tissue) was significantly increased from day 8 to day 60 of the experiment. The significantly lower volume of the ductal system (per mm3 of the tissue) was noted only 60 days after orchiectomy. The statistically significant shortening of the intralobular ducts (per mm3 of the tissue) was found from day 8 to day 60 after the operation. Interlobular ducts were significantly shorter from day 8 to day 15 after orchiectomy. The shortening of the rat parotid duct system and the changes of the parotid acini and the connective tissue after orchiectomy may be provoked by a lack of testosterone or some other substance which depends on androgens, such as epidermal growth factor (EGF).
Tijekom ovog istraživanja proučavana je struktura acinusa, kanalnog sustava i veziva zaušne žlijezde štakora nakon različitih razdoblja po odstranjenju muške spolne žlijezde (orhidektomije). Zreli štakori soja Fisher usmrćeni su 12 sati te 3, 8, 15, 30 i 60 dana poslije orhidektomije. Nakon odstranjenja zaušnih žlijezdi, histološki rezovi su stereološki analizirani pomoću Weibel-ove mnogonamjeneske testne mrežice. U usporedbi s kontrolom, volumen acinusa zaušne žlijezde (u mm3 tkiva) orhidektomiranih štakora bio je značajno snižen, dok je volumen vezivnog tkiva (u mm3 tkiva) bio značajno povećan od 8-og pa sve do 60-og dana pokusa. Značajno smanjenje volumena kanalnog sustava (u mm3 tkiva) moglo se otkriti tek 60 dana nakon orhidektomije. Morfometrijska analiza također je pokazala da dolazi do značajnog skraćenja intralobularnog kanalnog sustava (u mm3 tkiva) u razdoblju od 8-og do 60-og dana nakon operacije. Skraćenje interlobularnih kanala moglo se zabilježiti u orhidektomiranih štakora usmrćenih samo 8-og i 15-og dana pokusa. Ovo skraćenje kanalnog sustava kao i promjene na acinusima i vezivnom tkivu zaušne žlijezde štakora mogli bi biti uzrokovani nedostatkom testosterona ili neke druge tvari koja je ovisna o androgenima, kao npr. epidermalnog čimbenika rasta (EGF).
Databáze: OpenAIRE
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