VAŽNOST ODREĐIVANJA HPV INFEKCIJE I PROTEINA P16INK4a U CERVIKALNIM INTRAEPITELNIM NOVOTVORINAMA I INVAZIVNIM KARCINOMIMA CERVIKSA

Autor: Lidija Pejković, Irena Jeličić, Snježana Tomić, Marko Mimica, Irena Drmić Hoffman
Jazyk: chorvatština
Rok vydání: 2008
Předmět:
Zdroj: Gynaecologia et perinatologia : journal for gynaecology, perinatology, reproductive medicine and ultrasonic diagnostics
Volume 17
Issue 1
ISSN: 1330-0091
Popis: Cilj istraživanja. Utvrditi postoji li razlika u ekspresiji proteina p16INK4a u uzorcima nepromijenjenog, metaplastičnog i atrofičnog epitela te u različitim stupnjevima epitelne displazije i invazivnom karcinomu cerviksa, te postoji li povezanost između ekspresije proteina proteina p16INK4a i infekcije humanim papiloma virusom (HPV), poglavito infekcije visoko rizičnim sojevima HPV-a. Materijal i metode. Imunoihistokemijski je analizirana ¬pojavnost proteina p16 INK4a u 50 bioptičkih uzoraka vrata maternice, od čega 10 uzoraka neneoplastičnog epitela te po 10 uzoraka različitog stupnja displazije i invazivnog karcinoma cerviksa. Na svim uzorcima prethodno je napravljeno testiranje na prisutnost HPV DNK. Rezultati. Ni u jednom od uzoraka vrata maternice na čijoj površini se nalazio nepromijenjeni, metaplastični, ili atrofični epitel, nije nađena ni HPV infekcija ni pozitivno bojenje na protein p16INK4a. Od 10 uzoraka u kojih je postavljena dijagnoza CIN 1, u sedam je dokazana HPV infekcija, a samo su dva bila pozitivna nakon bojanja s p16INK4a antitijelom. U oba ova uzorka dokazan je HPV visokog stupnja rizika (VR HPV). Od10 uzoraka s dijagnozom CIN 2, VR HPV je dokazan u sedam i u svima je nađena pozitivna ekspresija proteina p16 INK4a. U dva od tih uzoraka u kojima nije dokazana HPV infekcija, nađeno je pozitivno bojanje na protein p16INK4a. Analizom 10 uzoraka s dijagnozom CIN 3, VR HPV je nađen u osam i u svima je nađena pozitivna ekspresija proteina p16INK4a. U jednom od dva HPV negativna uzorka nađeno je pozitivno bojanje na p16INK4a. Analizom 10 uzoraka s dijagnozom invazivnog karcinoma cerviksa, u sedam u kojima je postojao VR HPV, nađeno je pozitivno bojanje na p16INK4a. Pozitivno bojanje na protein nađeno je i u dva od tri negativna uzorka. Zaključak. Protein p16INK4a se ne pojavljuje u epitelu bez displastičnih promjena, a učestalost pojavljivanja mu se povećava sa stupnjem displazije. Porastom stupnja displazije raste i učestalost infekcije HPV-om visokog stupnja rizika. Imunohistokemijska detekcija proteina p16INK4a u bioptičkim uzorcima cerviksa je dobar indikator infekcije HPV-om visokog stupnja rizika, iako postoje pojedinačni uzorci u kojima je imunohistokemijski dokazan p16INK4a a nije dokazana virusna infekcija. Prema rezultatima ovog istraživanja, imunohistokemijsko bojanje na protein p16INK4a pokazuje svoju vrijednost u interpretaciji bioptičkih cervikalnih uzoraka. Ovo je preliminarni rad, te su prikazani rezultati dio većeg istraživanja koje slijedi.
Aim. To establish the existence of a difference between the p16INK4a protein expression in the samples of unchanged, metaplastic and atrophic cervical epithelial tissue and in different levels of epithelial dysplasia and invasive cervical carcinoma, as well as to establish whether there is a connection between the p16INK4a protein expression and the Human Papilloma Virus (HPV) infection, mainly from the high-risk group. Material and methods. The appearance of p16INK4a protein in 50 bioptic samples of cervix has been analysed, out of which 10 samples have been of non-neoplastic cervical epithelial tissue, and 10 samples of each of three cervical dysplasia level, as well as of invasive cervical carcinoma. All samples were previously tested for HPV DNA. Results. Not one of cervical samples with unchanged, metaplastic and atrophic epithelial tissue, contained either HPV infection or a positive staining on p16INK4a protein. Out of 10 samples which had CIN 1 diagnosis, 7 were proven to be HPV infected, and only 2 were positive after the colouring on p16INK4a. Both of these samples were proven to contain high-risk HPV infection (HR HPV). Out of 10 samples which had CIN 2 diagnosis, 7 were proven to contain HR HPV and in all of these samples a positive p16INK4a protein expression was found. In two out of three samples which were not proven to be HPV infected, there was a positive staining on p16INK4a protein. Analysing the samples which had CIN 3 diagnosis, HR HPV was found in 8, and in all of these samples a positive p16INK4a protein expression was found. In one out of two HPV negative samples a positive staining on p16INK4a protein was found. In the analysis of 10 samples with invasive cervical carcinoma diagnosis, in 7 containing HR HPV a positive staining on p16INK4a protein was found. A positive staining on p16INK4a protein was also found in two out of three HPV negative samples. Conclusion. The p16INK4a protein does not appear in the epithelial tissue without dysplasia changes, and the frequency of its appearance increases with the dysplasia grade. With the increase in the dysplasia level, the frequency of HR HPV infection gets higher. The p16INK4a protein is a good virus-infection indicator, although there are cases of this protein’s appearance without the infection. According to the results of this research, immunohistochemical colouring on p16INK4a protein shows its value in the cytological and bioptical cervical samples’ interpretation. This is a preliminary work and displays the results of the majority of the incoming research.
Databáze: OpenAIRE
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