Korelacija ekspresije TGF-β i MMP2 između adenokarcinoma prostate i okolnog nezahvaćenog parenhima
| Autor: | Mašić, Silvija, Bacalja, Jasna, Vučić, Majda, Čupić, Hrvoje, Tomas, Davor, Ulamec, Monika, Spajić, Borislav, Skenderi, Faruk, Krušlin, Božo |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Acta clinica Croatica Volume 61. Issue Supplement 3 |
| ISSN: | 1333-9451 0353-9466 |
| Popis: | In prostate adenocarcinoma, both tumorous stroma and epithelium have important role in tumor progression. Transforming growth factor beta (TGF- β) is a promotor in advanced stages of prostate cancer. Matrix Metalloproteinase 2 (MMP2), the endopeptidase that degrades extracellular matrix is considered to be overexpressed in prostatic carcinoma related to its growth and aggressiveness. Therefore, the aim was to analyze the expression of proteins TGF- β and MMP2 between both epithelium and stroma of prostatic adenocarcinoma and adjacent unaffected parenchyma. The intensity of TGF- β and MMP2 expression in epithelium, tumorous stroma and adjacent unaffected parenchyma was analyzed in 62 specimens of prostatic adenocarcinoma by microarray-based immunohistochemistry. TGF- β was more expressed in tumorous than in prostate stroma (p =0.000), while no statistical significance in case of MMP2 (p = 0.097) was found. MMP2 was more expressed in tumorous than in prostate epithelium (p =0.000), while no statistical significance in case of TGF- β (p = 0.096) was observed. The study results indicate that both tumorous stroma and epithelium have a role in tumor progression and support potential role of TGF- β and MMP2 in prostatic adenocarcinoma progression. U progresiji karcinoma prostate tumorska stroma i epitel imaju važnu ulogu. Beta transformirajući faktor rasta (TGF- β) djeluje kao promotor u uznapredovalom raku prostate, a matriks metaloproteinaza 2 (MMP2) kao endopeptidaza koja razgrađuje izvanstanični matriks te je njezina aktivnost pojačano izražena te povezana s rastom i agresivnošću ovog tumora. Stoga je cilj ovog istraživanja bio analizirati izraženost TGF- β u MMP2 između epitela i strome karcinoma prostate te okolnog tumorom nezahvaćenog parenhima prostate. Intenzitet izraženosti TGF- β i MMP2 u tumorskom epitelu i stromi te okolnom nezahvaćenom parenhimu je analiziran u 62 pacijenta s adenokarcinomom prostate metodom imunohistokemijske analize na uzorcima microarray-a. TGF- β je bio izraženiji u tumorskoj nego u ne- tumorskoj stromi (p =0.000), dok u slučaju izraženosti MMP2 nije pokazana statistička značajnost (p = 0.097). MMP2 je bila izraženija u tumorskom epitelu u odnosu na ne- tumorski epitel (p =0.000), dok statistička značajnost u slučaju izraženosti TGF- β nije pokazana (p = 0.096). Rezultati ovog istraživanja pokazuju da i tumorski epitel i stroma imaju ulogu u progresiji tumora i ukazuju na potencijalnu ulogu TGF- β i MMP2 u progresiji adenokarcinoma prostate. |
| Databáze: | OpenAIRE |
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